J Korean Rheum Assoc.  1995 Jan;2(1):34-41.

Detection Of Antibodies To Ena In The Systemic Lupus Erythematosus By Immunoblotting: A Cornparision Of Irnmunoblotting And Double Immunodiffusion

• Park SH
• Lee SH
• Cho CS
• Kim HY

Abstract

OBJECTIVE
Systemic Lupus Erythematosus(SLE) is characterized by various autoantibodies to a variety of nuclear antigens. Certain extractable nuclear antigens(ENA) have been served as extremly useful aids in differentiation of clinical subset, diagnostic marker and in the detection of early forms of systemic rheumatic diseases. This study was to employ immunoblot to determine the prevalences of antibodies to ENA in SLE compared with immunodiffusion.
METHODS
Sera were obtained from 127 SLE patients. Antibodies to ENA were assessed by double immunodiffusion (DID) and immunoblot method.
RESULTS
Using the immunblot method, the prevalences of antibodies to ENA were as follows: The antibody to Sm was 27%, UIRNP 33.6%, Ro 41.8%, La 14%, ribosomal P 14% and Ku 4%. The prevalences of antibodies to ENA by DID were as follows: The antibody to Sm was 15%, RNP 24.5%, Ro 54.3% and La 9.4%.
CONCLUSIONS
Compared with immunodiffusion, results using immunoblot showed greater sensitivity in the detection of autoantibodies to ENA in SLE.

Keyword

systemic lupus erythematosus; ENA; Immunoblot

MeSH Terms

Antibodies*
Antigens, Nuclear
Autoantibodies
Humans
Immunoblotting*
Immunodiffusion*
Lupus Erythematosus, Systemic*
Prevalence
Rheumatic Diseases
Antibodies
Antigens, Nuclear
Autoantibodies