J Korean Assoc Maxillofac Plast Reconstr Surg.  2011 Jul;33(4):301-307.

Response of Odontoblast to the Bio-Calcium Phosphate Cement

• Kim JW
• Kim SW
• Kim GC
• Kim YD
• Kim CH
• Kim BJ
• Kim UK

Abstract

PURPOSE
If the tooth structure is damaged, then it is impossible to regenerate the tooth. The materials used to restore the tooth structure are not related to the composition of the tooth. The materials used to restore the structure can't replace the natural tooth because they just fill the defective structure. Calcium phosphate cement remineralizes the dentin and almost replaces the natural tooth, but there are some disadvantages. We conducted basic tests with Biomimetic CPC (Bio-CPC) to make sure of the possibility of the biomaterial to remineralize the defective tooth structure.
METHODS
In this study, the bioactivity and biocompatibility of Bio-CPC were evaluated for its potential value as the bio-material for regeneration of damaged tooth structure by conducting a cell toxicity assay (WST-1 assay), a cytokinesis-block micronucleus assay, a chromosomal aberration test, total RNA extraction and RT-PCR on MDPC-23 mouse odontoblast-like cells.
RESULTS
The in vitro cytotoxicity test showed that the Bio-CPC was fairly cytocompatible for the MDPC-23 mouse odontoblast-like cells.
CONCLUSION
Bio-CPC has a possibility to be a new biomaterial and further study of Bio-CPC is needed.

Keyword

Calcium phosphate cement (CPC); Odontoblast

MeSH Terms

Animals
Biomimetics
Calcium
Calcium Phosphates
Chromosome Aberrations
Dentin
Dinucleoside Phosphates
Mice
Micronucleus Tests
Odontoblasts
Regeneration
RNA
Tooth
Calcium
Calcium Phosphates
Dinucleoside Phosphates
RNA