Korean J Nephrol.
1999 Jul;18(4):523-529.
Tissue-Specific Regulation of Angiotensinogen and Angiotensin II Receptor Genes in Two-Kidney, One Clip Hypertensive Rats
- Affiliations
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- 1Department of Physiology, Chonnam University Medical School, Korea.
- 2Department of Oral Biology, Chonnam University College of Dentistry, Kwangju, Korea.
Abstract
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The present study was aimed at investigating the molecular regulation of the renin- angiotensin
system (RAS) in two-kidney, one clip (2K1C) hypertension. The expression of renin,
angiotensinogen and angiotensin II receptor genes was determined by Northern blot analysis
in rats made 2K1C hypertensive for 2 or 4 weeks. The expression of renin gene was increased
in the clipped kidney and decreased in the contralateral non-clipped kidney at weeks 2 and 4.
The expression of angiotensinogen gene was not significantly altered at week 2, but increased
at week 4 in the clipped kidney. However, it was not significantly altered in the contralateral
kidney either at week 2 or 4. Nor was the expression of angiotensinogen gene significantly
altered in the liver either at week 2 or 4. On the other hand, the expression of
angiotensin II receptor gene was decreased at week 2, and increased at week 4 in the clipped
kidney, whereas it was not significantly changed in the contralateral kidney either at
week 2 or 4. In the liver, the expression of angiotensin II receptor gene was not
significantly altered at week 2, but decreased at week 4. These results suggest that
the components of RAS are transcriptionally regulated in 2K1C hypertension in a manner
dependent on tissues and duration of hypertension.