Korean J Nephrol.  1999 Jul;18(4):523-529.

Tissue-Specific Regulation of Angiotensinogen and Angiotensin II Receptor Genes in Two-Kidney, One Clip Hypertensive Rats

Affiliations
  • 1Department of Physiology, Chonnam University Medical School, Korea.
  • 2Department of Oral Biology, Chonnam University College of Dentistry, Kwangju, Korea.

Abstract

The present study was aimed at investigating the molecular regulation of the renin- angiotensin system (RAS) in two-kidney, one clip (2K1C) hypertension. The expression of renin, angiotensinogen and angiotensin II receptor genes was determined by Northern blot analysis in rats made 2K1C hypertensive for 2 or 4 weeks. The expression of renin gene was increased in the clipped kidney and decreased in the contralateral non-clipped kidney at weeks 2 and 4. The expression of angiotensinogen gene was not significantly altered at week 2, but increased at week 4 in the clipped kidney. However, it was not significantly altered in the contralateral kidney either at week 2 or 4. Nor was the expression of angiotensinogen gene significantly altered in the liver either at week 2 or 4. On the other hand, the expression of angiotensin II receptor gene was decreased at week 2, and increased at week 4 in the clipped kidney, whereas it was not significantly changed in the contralateral kidney either at week 2 or 4. In the liver, the expression of angiotensin II receptor gene was not significantly altered at week 2, but decreased at week 4. These results suggest that the components of RAS are transcriptionally regulated in 2K1C hypertension in a manner dependent on tissues and duration of hypertension.

Keyword

Renin; Angiotensinogen; Angiotensin II receptor; Northern blot analysis; Two-kidney; One clip hypertension

MeSH Terms

Angiotensin II*
Angiotensinogen*
Angiotensins*
Animals
Blotting, Northern
Hand
Hypertension
Kidney
Liver
Rats*
Receptors, Angiotensin*
Renin
Angiotensin II
Angiotensinogen
Angiotensins
Receptors, Angiotensin
Renin
Full Text Links
  • KJN
Actions
Cited
CITED
export Copy
Close
Share
  • Twitter
  • Facebook
Similar articles
Copyright © 2024 by Korean Association of Medical Journal Editors. All rights reserved.     E-mail: koreamed@kamje.or.kr