Endocrinol Metab.  2012 Dec;27(4):276-281. 10.3803/EnM.2012.27.4.276.

APOA5 Polymorphism Is Associated with Metabolic Syndrome in Korean Postmenopausal Women

Affiliations
  • 1Research Institute, Seoul Medical Center, Seoul, Korea.
  • 2Department of Neurosurgery, Seoul Medical Center, Seoul, Korea.
  • 3Department of Obstetrics and Gynecology, Seoul Medical Center, Seoul, Korea.
  • 4Department of Internal Medicine, Seoul Medical Center, Seoul, Korea. people@chol.com

Abstract

BACKGROUND
Menopause is an independent risk factor in metabolic syndrome which induced an alteration of the lipid metabolism by hormonal changes. Apolipoprotein A5 gene (APOA5) was related to the regulation of triglyceride and high density lipoprotein cholesterol (HDL-C) level with biosynthesis and decomposition. This study was conducted to investigate the relationship between APOA5 polymorphism and metabolic syndrome in Korean postmenopausal women.
METHODS
This study included 307 postmenopausal women with anthropometric and biochemical measurement in 2010-2011. The polymorphism of APOA5 was analyzed by polymerase chain reaction-restriction fragment length polymorphism method with MseI restriction enzyme.
RESULTS
The metabolic syndrome prevalence with TT genotype was significantly lower than the frequency in those with TC/CC (27.09%, 38.46%, and 45.71% for TT, TC, and CC, respectively; P < 0.05). Multiple regression analysis of metabolic syndrome risk factors indicated that postmenopausal women with CC genotype had a higher risk with 3 times than that in TT genotype (P < 0.05). APOA5 C carriers showed an increased risk of triglyceride level (odd ratio, 2.93 and 1.85 for CC and TC+CC, respectively; P < 0.05). Interestingly, HDL-C was related to triglyceride directly in comparison to APOA5.
CONCLUSION
The results of this study indicate that APOA5 has an influence on serum triglyceride and HDL-C, which contribute to metabolic syndrome in Korean postmenopausal women.

Keyword

Apolipoproteins A; HDL cholesterol; Metabolic syndrome X; Single nucleotide polymorphism; Triglycerides

MeSH Terms

Apolipoproteins
Apolipoproteins A
Cholesterol
Cholesterol, HDL
Female
Genotype
Humans
Lipid Metabolism
Lipoproteins
Menopause
Metabolic Syndrome X
Polymorphism, Single Nucleotide
Prevalence
Risk Factors
Triglycerides
Apolipoproteins
Apolipoproteins A
Cholesterol
Cholesterol, HDL
Lipoproteins
Triglycerides

Figure

  • Fig. 1 The prevalence of metabolic syndrome according to the APOA5 isoforms. *P < 0.05 by chi-square test. APOA5, apolipoprotein A5; MetS, metabolic syndrome.


Reference

1. Royer M, Castelo-Branco C, Blumel JE, Chedraui PA, Danckers L, Bencosme A, Navarro D, Vallejo S, Espinoza MT, Gomez G, Izaguirre H, Ayala F, Martino M, Ojeda E, Onatra W, Saavedra J, Tserotas K, Pozzo E, Manriquez V, Prada M, Grandia E, Zuniga C, Lange D, Sayegh F. Collaborative Group for Research of the Climacteric in Latin America. The US National Cholesterol Education Programme Adult Treatment Panel III (NCEP ATP III): prevalence of the metabolic syndrome in postmenopausal Latin American women. Climacteric. 2007. 10:164–170.
2. Hidalgo LA, Chedraui PA, Morocho N, Alvarado M, Chavez D, Huc A. The metabolic syndrome among postmenopausal women in Ecuador. Gynecol Endocrinol. 2006. 22:447–454.
3. Ushiroyama T, Sakuma K, Ikeda A, Ueki M. The HDL2/HDL3 ratio in menopause. Int J Gynaecol Obstet. 2005. 88:303–308.
4. Janssen I, Powell LH, Crawford S, Lasley B, Sutton-Tyrrell K. Menopause and the metabolic syndrome: the Study of Women's Health Across the Nation. Arch Intern Med. 2008. 168:1568–1575.
5. Anand SS, Yi Q, Gerstein H, Lonn E, Jacobs R, Vuksan V, Teo K, Davis B, Montague P, Yusuf S. Relationship of metabolic syndrome and fibrinolytic dysfunction to cardiovascular disease. Circulation. 2003. 108:420–425.
6. Fasshauer M, Paschke R. Regulation of adipocytokines and insulin resistance. Diabetologia. 2003. 46:1594–1603.
7. Silha JV, Krsek M, Skrha JV, Sucharda P, Nyomba BL, Murphy LJ. Plasma resistin, adiponectin and leptin levels in lean and obese subjects: correlations with insulin resistance. Eur J Endocrinol. 2003. 149:331–335.
8. Li GP, Wang JY, Yan SK, Chen BS, Xue H, Wu G. Genetic effect of two polymorphisms in the apolipoprotein A5 gene and apolipoprotein C3 gene on serum lipids and lipoproteins levels in a Chinese population. Clin Genet. 2004. 65:470–476.
9. Lee KH, Kim OY, Lim HH, Lee YJ, Jang Y, Lee JH. Contribution of APOA5-1131C allele to the increased susceptibility of diabetes mellitus in association with higher triglyceride in Korean women. Metabolism. 2010. 59:1583–1590.
10. Song KH, Yu SG, Cha S, Kim JY. Association of the apolipoprotein A5 gene -1131T>C polymorphism with serum lipids in Korean subjects: impact of Sasang Constitution. Evid Based Complement Alternat Med. 2012. 2012:598394.
11. Nabika T, Nasreen S, Kobayashi S, Masuda J. The genetic effect of the apoprotein AV gene on the serum triglyceride level in Japanese. Atherosclerosis. 2002. 165:201–204.
12. Yamada Y, Kato K, Hibino T, Yokoi K, Matsuo H, Segawa T, Watanabe S, Ichihara S, Yoshida H, Satoh K, Nozawa Y. Prediction of genetic risk for metabolic syndrome. Atherosclerosis. 2007. 191:298–304.
13. Cardona F, Guardiola M, Queipo-Ortuño MI, Murri M, Ribalta J, Tinahones FJ. The -1131T>C SNP of the APOA5 gene modulates response to fenofibrate treatment in patients with the metabolic syndrome: a postprandial study. Atherosclerosis. 2009. 206:148–152.
14. Grundy SM, Cleeman JI, Daniels SR, Donato KA, Eckel RH, Franklin BA, Gordon DJ, Krauss RM, Savage PJ, Smith SC Jr, Spertus JA, Costa F. American Heart Association, National Heart, Lung, and Blood Institute. Diagnosis and management of the metabolic syndrome: an American Heart Association/National Heart, Lung, and Blood Institute Scientific Statement. Circulation. 2005. 112:2735–2752.
15. Pan WH, Yeh WT, Weng LC. Epidemiology of metabolic syndrome in Asia. Asia Pac J Clin Nutr. 2008. 17:Suppl 1. 37–42.
16. Pennacchio LA, Olivier M, Hubacek JA, Cohen JC, Cox DR, Fruchart JC, Krauss RM, Rubin EM. An apolipoprotein influencing triglycerides in humans and mice revealed by comparative sequencing. Science. 2001. 294:169–173.
17. Szalai C, Keszei M, Duba J, Prohaszka Z, Kozma GT, Csaszar A, Balogh S, Almassy Z, Fust G, Czinner A. Polymorphism in the promoter region of the apolipoprotein A5 gene is associated with an increased susceptibility for coronary artery disease. Atherosclerosis. 2004. 173:109–114.
18. Song HO, Kim SS. A literature review of single nucleotide polymorphisms in obesity genes. J Korean Orient Assoc Stud Obes. 2004. 4:139–160.
19. Grallert H, Sedlmeier EM, Huth C, Kolz M, Heid IM, Meisinger C, Herder C, Strassburger K, Gehringer A, Haak M, Giani G, Kronenberg F, Wichmann HE, Adamski J, Paulweber B, Illig T, Rathmann W. APOA5 variants and metabolic syndrome in Caucasians. J Lipid Res. 2007. 48:2614–2621.
20. Kisfali P, Mohás M, Maász A, Polgár N, Hadarits F, Markó L, Brasnyó P, Horvatovich K, Oroszlán T, Bagosi Z, Bujtor Z, Gasztonyi B, Rinfel J, Wittmann I, Melegh B. Haplotype analysis of the apolipoprotein A5 gene in patients with the metabolic syndrome. Nutr Metab Cardiovasc Dis. 2010. 20:505–511.
21. Maasz A, Kisfali P, Horvatovich K, Mohás M, Markó L, Csöngei V, Faragó B, Járomi L, Magyari L, Sáfrány E, Sipeky C, Wittmann I, Melegh B. Apolipoprotein A5 T-1131C variant confers risk for metabolic syndrome. Pathol Oncol Res. 2007. 13:243–247.
22. Park HS, Oh SW, Cho SI, Choi WH, Kim YS. The metabolic syndrome and associated lifestyle factors among South Korean adults. Int J Epidemiol. 2004. 33:328–336.
23. Park YW, Zhu S, Palaniappan L, Heshka S, Carnethon MR, Heymsfield SB. The metabolic syndrome: prevalence and associated risk factor findings in the US population from the Third National Health and Nutrition Examination Survey, 1988-1994. Arch Intern Med. 2003. 163:427–436.
24. Carr MC. The emergence of the metabolic syndrome with menopause. J Clin Endocrinol Metab. 2003. 88:2404–2411.
25. Kim SM, Kim SH, Lee JR, Jee BC, Ku SY, Suh CS, Choi YM, Kim JG, Moon SY. The effects of hormone therapy on metabolic risk factors in postmenopausal Korean women. Climacteric. 2011. 14:66–74.
26. Jang Y, Paik JK, Hyun YJ, Chae JS, Kim JY, Choi JR, Lee SH, Shin DJ, Ordovas JM, Lee JH. The apolipoprotein A5 -1131T>C promoter polymorphism in Koreans: association with plasma APOA5 and serum triglyceride concentrations, LDL particle size and coronary artery disease. Clin Chim Acta. 2009. 402:83–87.
27. Cabré A, Lázaro I, Girona J, Manzanares JM, Marimón F, Plana N, Guardiola M, Heras M, Masana L. The APOA5-1131 T>C variant enhances the association between RBP4 and hypertriglyceridemia in diabetes. Nutr Metab Cardiovasc Dis. 2010. 20:243–248.
28. Jang Y, Kim JY, Kim OY, Lee JE, Cho H, Ordovas JM, Lee JH. The -1131T→C polymorphism in the apolipoprotein A5 gene is associated with postprandial hypertriacylglycerolemia: elevated small, dense LDL concentrations: and oxidative stress in nonobese Korean men. Am J Clin Nutr. 2004. 80:832–840.
29. Sane T, Nikkilä EA, Taskinen MR, Välimäki M, Ylikahri R. Accelerated turnover of very low density lipoprotein triglycerides in chronic alcohol users. A possible mechanism for the up-regulation of high density lipoprotein by ethanol. Atherosclerosis. 1984. 53:185–193.
30. Aouizerat BE, Kulkarni M, Heilbron D, Drown D, Raskin S, Pullinger CR, Malloy MJ, Kane JP. Genetic analysis of a polymorphism in the human apoA-V gene: effect on plasma lipids. J Lipid Res. 2003. 44:1167–1173.
31. Evans D, Buchwald A, Beil FU. The single nucleotide polymorphism -1131T>C in the apolipoprotein A5 (APOA5) gene is associated with elevated triglycerides in patients with hyperlipidemia. J Mol Med (Berl). 2003. 81:645–654.
32. Hadarits F, Kisfali P, Mohás M, Maász A, Duga B, Janicsek I, Wittmann I, Melegh B. Common functional variants of APOA5 and GCKR accumulate gradually in association with triglyceride increase in metabolic syndrome patients. Mol Biol Rep. 2012. 39:1949–1955.
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