J Korean Soc Microbiol.  1999 Apr;34(2):157-162.

Isolation of Echovirus Serotype 25 from Patient with Hand , Foot and Mouth Disease in Pusan , 1998

• Kim YH
• Cho KS

Abstract

Identification of virus is very importnant in aspects of preventive surveillance system of viral infection. The first isolation of echovirus serotype 25 from hand, foot and mouth disease was accomplished in Pusan, 1998. The authors determined some properties of this virus. Two cases of outbreak were confirmed from the stools of a 3 year-old girl and one 4 year-old boy suffering from hand, foot and mouth disease. Both occurred in April. The isolated viruses showed strong cytopathic effect on RD cells, also on HEp-2, and Vero cell lines after 3 days at 34'C, CO incubation. Isolated virus was identified as echovirus serotype 25 by neutralizing antibody test. Electron micrograph of negative-stained echovirus serotype 25 showed non-enveloped, isometric particle and about 30 nm in diameter.

MeSH Terms

Animals
Antibodies, Neutralizing
Busan*
Child, Preschool
Enterovirus B, Human*
Female
Foot*
Foot-and-Mouth Disease*
Hand*
Hand, Foot and Mouth Disease
Humans
Male
Vero Cells
Antibodies, Neutralizing