Korean J Fertil Steril.  1997 Apr;24(1):83-93.

Microassisted Fertilization of Human Oocytes with Intracytoplasmic Sperm Injection in IVF-ET Patients with History of Failure in Fertilization or Extremely Low Fertilization Rate in Previous Cycles

• No authors listed

Abstract

Although IVF-ET is widely applied in the treatment of couples with male factor infertility, it may fail in many infertile couples with normal semen parameters, and certain couples cannot be accepted for standard IVF-ET due to unfertilization or extremely low fertilization rate of oocytes. Recently, several procedures of microassisted fertilization (MAF) using micromanipulation have been introduced, and pregnancies and births have been obtained after partial zona dissection (PZD), subzonal insertion (SUZI), and intracytoplasmic sperm injection (ICSI). This clinical study was performed to develop and establish ICSI as an effective procedure of MAF in infertile couples who could not undergo standard IVF-ET repetitively because of failure in fertilization or extremely low fertilization rate of oocytes with the conventional fertilization technique in the previous IVF-ET cycles. From March, 1995 to May, 1996, 27 cycles of IVF-ET with ICSI in 19 infertile patients were included in study group, and the outcomes of ICSI were analyzed according to fertilization rate, cumulative embryo score (CES), and pregnancy rate. The number of oocytes retrieved after controlled ovarian hyperstimulation (COH) was 10.50+/-6.13 in 30 previous cycles, and 10.57+/-5.53 in 27 ICSI cycles. In ICSI cycles, the number of oocytes optimal for ICSI procedure was 7.89+/-4.30, and the fertilization rate of 67.9+/-20.2% could be obtained after ICSI. The number of embryos transferred was 1.43+/-2.40 in previous cycles, and 4.36+/-1.77 with the mean CES of 41.8+/-27.4 in ICSI cycles. In ICSI cycles, the overall pregnancy rate was 29.6% (8/27) per cycle and 42.1% (8/19) per patient with the clinical pregnancy rate of 22.2% (6/27) per cycle and 31.6% (6/19) per patient. In conclusion, MAF of human oocytes with ICSI is a promising fertilization method for IVF-ET patients, especially with the past history of failure in fertilization or low fertilization rate of oocytes in the previous IVF-ET cycles, and ICSI using micromanipulation procedures applied to human oocytes will provide a range of novel techniques which may dramatically improve the pregnancy rate in IVF-ET program and contribute much to effective management of infertile couples.

MeSH Terms

Embryonic Structures
Family Characteristics
Fertilization*
Herpes Zoster
Humans*
Infertility
Male
Micromanipulation
Oocytes*
Parturition
Pregnancy
Pregnancy Rate
Semen
Sperm Injections, Intracytoplasmic*