Korean J Obstet Gynecol.  2002 Aug;45(8):1289-1293.

Effects of Glutathione on Cisplatin-Induced Cytotoxicity In Human Cervical Cancer Cell Lines

Affiliations
  • 1Department of Obstetrics and Gynecology, College of Medicine, Chosun University, Kwangju, Korea.

Abstract


OBJECTIVE
The purpose of this study is to determine the effects of glutathione on cisplatin-induced cytotoxicity of human cervical carcinoma cell lines (SiHa: squamous cell carcinoma cell, CaSki: epidermoid metastatic carcinoma cell).
METHODS
Human cervical carcinoma cells (SiHa, CaSki) were incubated with culture media (RPMI1640) in the presence of cisplatin and/or buthionine sulfoximine (BSO), as a inhibitor of gamma-glutamyl- cysteine synthetase, and/or glutathione (GSH) and/or 2-oxo 4-thiazolidine carboxylic acid (OTC). The viable cells were examined by using 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide (MTT) and was determined by spectrophotometer at 570 nm.
RESULTS
The incubation of cervical cells with cisplatin resulted in an decreasing cells viability by dose response. The MTT reduction rate were not different by BSO (5 mM) treatment in cervical cell lines. The viable cells were increased significantly by glutathione (5 mM) or OTC (5 mM) in cisplatin-treated cell lines.
CONCLUSION
gamma-glutamylcysteine synthetase inhibitor had no effect on cisplatin toxicity. GSH and OTC had effect on cisplatin cytotoxicity. So, These result suggested that cervical cancer line cells were more susceptive to protective effects of glutathione and OTC than BSO on cisplatin induced-toxicity.

Keyword

Cisplatin; Cervical carcinoma cell; Glutathione; OTC

MeSH Terms

Buthionine Sulfoximine
Carcinoma, Squamous Cell
Cell Line*
Cisplatin
Culture Media
Cysteine
Glutathione*
Humans*
Ligases
Uterine Cervical Neoplasms*
Buthionine Sulfoximine
Cisplatin
Culture Media
Cysteine
Glutathione
Ligases
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