J Korean Orthop Res Soc.  2002 Apr;5(1):55-60.

In Vitro Release of Vancomycin from Vancomycin-loaded Demineralized Bone Coated with Hematoma

• Rhyu KH
• Lim ST
• Kim HJ

Abstract

PURPOSE: This study was performed to find a method to utilize demineralized bone matrix as a vehicle for local delivery of the antibiotics for the treatment of chronic osteomyelitis.
MATERIALS AND METHODS
Bovine bone blocks with the same size were randomly divided into 4 groups. Using Demineralization and hematoma coating, the different characteristics were given to each group, and Vancomycin was adsorbed to these bone blocks. We performed two identical experiments with Phosphate-buffered Saline and Fetal Bovine Serum as a solute. We exchange the solution with 24-hour interval for 6 weeks. The eluted concentrations of Vancomycin at each week were measured.
RESULTS
Regardless of the solution used, the eluted concentration of Vancomycin was significantly higher in demineralized and hematoma-coated group than those of other groups. The eluted concentration of Vancomycin were 22.6ng/ml at week 6 in saline condition and 12.4ng/ml at week 5 in serum condition.
CONCLUSIONS
We thought that demineralized and hematoma-coated cancellous bone can be used as a good vehicle for local antibiotics delivery.

Keyword

Cancellous Bone; Demineralized; Hematoma Coating

MeSH Terms

Anti-Bacterial Agents
Bone Matrix
Hematoma*
Osteomyelitis
Vancomycin*
Anti-Bacterial Agents
Vancomycin