Korean J Otolaryngol-Head Neck Surg.  1999 May;42(5):610-616.

Distribution and Origin of Nitrergic Innervation of the Canine Laryngeal Ventricle

• Kim HT
• Cho SH

Abstract

BACKGROUND AND OBJECTIVES: Recently, nitric oxide (NO) has been considered to be a neurotransmitter or a signaling molecule in a number of distinct subpopulation of neurons in the central and peripheral nervous systems. This study attempted to define the distribution patterns and quantitative participation according to the origin of nitrergic innervation in the canine laryngeal ventricles of eight adult dogs.
MATERIALS AND METHODS
The nitrergic innervation in the intralaryngeal, superior cervical and nodose ganglion to the laryngeal ventricle were investigated by using double labelled neuronal NO synthase (nNOS) immunocytochemistry combined with a retrograde tracer, cholera toxin subunit B-conjugated horseradish peroxidase (CTB-HRP).
RESULTS
NO is suggested to participate in parasympathetic, sympathetic and sensory innervation. Intralaryngeal ganglion is the main source of nitrergic innervation in the canine laryngeal ventricle. The proportions of the nitrergic innervation in the intralaryngeal ganglion, superior cervical ganglion, and nodose ganglion to the canine laryngeal ventricle were 63.1%, 37.7%, 4.9% respectively.
CONCLUSION
NO originating from the intralaryngeal ganglion in a canine laryngeal ventricle may play an important role in controlling the laryngeal gland secretion and in regulating the blood flow by modulating the classical parasympathetic cholinergic neurotransmitter as like a neuronal messenger or comediator. NO also may participate in the same role through the sympathetic innervation of superior cervical ganglion: however, NO originating from intralaryngeal ganglion may play more important role than that from superior cervical ganglion. Many neurons of nodose ganglion have demonstrated to have nNOS, but might be less involved in the ventricular sensory innervation.

Keyword

Neuronal nitric oxide synthase; Laryngeal ventricle; Intralaryngeal ganglion; Superior cervical ganglion; Nodose ganglion

MeSH Terms

Adult
Animals
Cholera Toxin
Dogs
Ganglion Cysts
Horseradish Peroxidase
Humans
Immunohistochemistry
Neurons
Neurotransmitter Agents
Nitric Oxide
Nitric Oxide Synthase
Nitric Oxide Synthase Type I
Nodose Ganglion
Peripheral Nervous System
Superior Cervical Ganglion
Cholera Toxin
Horseradish Peroxidase
Neurotransmitter Agents
Nitric Oxide
Nitric Oxide Synthase
Nitric Oxide Synthase Type I