Korean Circ J.  2001 Feb;31(2):230-237. 10.4070/kcj.2001.31.2.230.

Effect of Ethanol on the Regulation of Smooth Muscle Tone in Rat Aorta

• Suh DJ
• Bae HR
• Jung DK
• Lee JH

• KMID: 1858589
• DOI: http://doi.org/10.4070/kcj.2001.31.2.230

Abstract

BACKGROUND AND OBJECTIVES: The aim of this study was to determine the effect of ethanol on the regulation of vascular tone. MATERIAL AND METHODS: Using rat aorta ring, isometric contraction and 45Ca uptake were measured. Phorbol 12,13-dibutyrate (PDBu), phenylephrine, KCl were used for the regulation of smooth muscle tone.
RESULTS
Ethanol induced transient contraction in rat aorta ring by dose-dependent manner. Ethanol suppressed the dose dependent contractile responses of vascular strip by phenylephrine, KCl and PDBu. Endothelium-dependent relaxation by acetylcholine was inhibited by ethanol. Ethanol depressed 45Ca uptake by high KCl but not by phenylephrine or PDBu in rat aorta. n-butanol selectively suppressed tonic contraction by high KCl, but t-butanol did not at the same concentration of butanol in rat aorta. PDBu-induced contraction was selectively suppressed by n-butanol but not by t-butanol.
CONCLUSIONS
These findings suggest that the action of ethanol on phospholipase D is involved in the decreased response of rat aorta strip by vasoconstrictors.

Keyword

ethanol; butanol; Protein Kinase C; Phospholipase D

MeSH Terms

1-Butanol
Acetylcholine
Animals
Aorta*
Ethanol*
Isometric Contraction
Muscle, Smooth*
Phenylephrine
Phorbol 12,13-Dibutyrate
Phospholipase D
Protein Kinase C
Rats*
Relaxation
tert-Butyl Alcohol
Vasoconstrictor Agents
1-Butanol
Acetylcholine
Ethanol
Phenylephrine
Phorbol 12,13-Dibutyrate
Phospholipase D
Protein Kinase C
Vasoconstrictor Agents
tert-Butyl Alcohol