Korean J Helicobacter Up Gastrointest Res.  2012 Jun;12(2):82-87. 10.7704/kjhugr.2012.12.2.82.

Analysis of Antimicrobial Resistance of Helicobacter pylori in Gwangju, Chonnam Provinces

Affiliations
  • 1Department of Internal Medicine, Chonnam National University Medical School, Gwangju, Korea. dshskim@jnu.ac.kr

Abstract

BACKGROUND/AIMS
A triple therapy, comprised of a proton pump inhibitor, clarithromycin, and amoxicillin or metronidazole, is the most commonly used treatment for Helicobacter pylori (H. pylori) eradication. But, H. pylori have become resistant to antimicrobial agents, reducing eradication rates. Thus, we investigated the distribution of minimal inhibitory concentration (MIC) for these drugs and analyzed the factors that affect it.
MATERIALS AND METHODS
From July 2008 through December 2009, we performed tissue culture with gastric mucosa from 51 patients who were infected with H. pylori and measured MIC by Epsilometer test (E-test).
RESULTS
The mean age of the group was 56.8+/-13.8 years, and the male to female ratio was 2:1. Resistance rates of H. pylori to amoxillin, clarithromycin, metronidazole, ciprofloxacin, levofloxacin, tetracycline were 2%, 15.7%, 21.6%, 25.5%, 25.5%, and 0%, respectively. Statistically, there were no significant relationship between resistance rate and other factors including age, sex, alcohol or smoking. Follow up test revealed eradication failure in 4 of 22 patients but, MIC for amoxillin and clarithromycin of these four patients were considered to belong to the susceptible group. Three patients belonging to the clarithromycin-resistant group (MIC>256 microg/mL), were eradicated. In addition, one of the three patients showed resistant to not only clarithromycin (MIC 12 microg/mL), but also amoxiciliin (MIC 0.6 microg/mL).
CONCLUSIONS
The microbial susceptability test of each antibiotics does not accurately reflecte successful eradication, but further investigation is needed to identify the factors that influence the eradication of H. pylori.

Keyword

Helicobacter pylori; Micribial sensitivity test

MeSH Terms

Amoxicillin
Anti-Bacterial Agents
Anti-Infective Agents
Ciprofloxacin
Clarithromycin
Female
Follow-Up Studies
Gastric Mucosa
Helicobacter
Helicobacter pylori
Humans
Male
Metronidazole
Ofloxacin
Proton Pumps
Smoke
Smoking
Tetracycline
Amoxicillin
Anti-Bacterial Agents
Anti-Infective Agents
Ciprofloxacin
Clarithromycin
Metronidazole
Ofloxacin
Proton Pumps
Smoke
Tetracycline
Full Text Links
  • KJHUGR
Actions
Cited
CITED
export Copy
Close
Share
  • Twitter
  • Facebook
Similar articles
Copyright © 2024 by Korean Association of Medical Journal Editors. All rights reserved.     E-mail: koreamed@kamje.or.kr