J Korean Soc Emerg Med.  2011 Aug;22(4):335-342.

Effect of Calcium Channel Blocker on Primary Rat Cardiomyoblasts and Cardiac Rate in Vitro

Affiliations
  • 1Department of Emergency Medicine, Wonkwang University College of Medicine, Iksan, Korea. ysoojin@wmc.wonkwang.ac.kr

Abstract

PURPOSE
This work was intended to establish experimental conditions for monitoring the effect of ischemic/reperfusion injury on the beating capability of, and apoptotic damage to, primary rat cardiomyoblasts, and to investigate the cardioprotective effect of calcium channel blocker on primary rat cardiomyoblasts with ischemic/reperfusion injury including reactive oxygen species damage.
METHODS
To generate ischemic/reperfusion injury, primary rat cardiomyoblasts were treated with hydrogen peroxide (H2O2). Injured cardiomyoblasts were pretreated with the calcium channel blocker nicardipine.
RESULTS
Treatment with H2O2 significantly decreased the cardiac rate of primary rat cardiomyoblasts in time- and dose-dependent manners. Interestingly, the cardiac rate of primary rat cardiomyoblasts abruptly dropped prior to the decrease in cell viability. H2O2 also induced a decrease in the expression of heme oxygenase-1 (HO-1) protein in P2 primary rat cardiomyoblasts in a time-dependent manner. Moreover, treatment with H2O2 resulted in an increase of DNA fragmentation, indicating that H2O2 induced the apoptotic death of P2 primary rat cardiomyoblasts. However, pretreatment with nicardipine markedly decreased cell death in H2O2-treated primary rat cardiomyoblasts by regulating HO-1 protein expression.
CONCLUSION
Nicardipine has a cardioprotective effect that helps maintain the viability of primary rat cardiomyoblasts with induced ischemic/reperfusion injury, including reactive oxygen species-induced damage.

Keyword

Calcium channel blocker; Hydrogen peroxide; Apoptosis; Ischemia

MeSH Terms

Animals
Apoptosis
Calcium
Calcium Channels
Cell Death
Cell Survival
DNA Fragmentation
Heme Oxygenase-1
Hydrogen Peroxide
Ischemia
Nicardipine
Oxygen
Rats
Reactive Oxygen Species
Calcium
Calcium Channels
Heme Oxygenase-1
Hydrogen Peroxide
Nicardipine
Oxygen
Reactive Oxygen Species
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