Yonsei Med J.  2013 Nov;54(6):1336-1341. 10.3349/ymj.2013.54.6.1336.

Inflammatory Responses in the Muscle Coat of Stomach and Small Bowel in the Postoperative Ileus Model of Guinea Pig

• Choi HK
• Lee YH
• Park JP
• Min K
• Park H

• KMID: 1798128
• DOI: http://doi.org/10.3349/ymj.2013.54.6.1336

Abstract

PURPOSE
Small intestinal function returns first after surgery, and then the function of the stomach returns to normal after postoperative ileus (POI). The aim of this study was to investigate inflammatory responses in the muscle coat of stomach and small intestine in guinea pig POI model.
MATERIALS AND METHODS
The distance of charcoal migration from pylorus to the distal intestine was measured. Hematoxylin and eosin (H&E) and immunohistochemical stain for calprotectin were done from the histologic sections of stomach, jejunum and ileum obtained at 3 and 6 hour after operation. Data were compared between sham operation and POI groups.
RESULTS
The distance of charcoal migration was significantly reduced in the 3 and 6 hour POI groups compared with sham operated groups (p<0.05). On H&E staining, the degree of inflammation was significantly higher in the stomach of 3 hour POI groups compared with jejunum and ileum of POI groups or sham operated groups (p<0.05). Calprotectin positive cells were significantly increased in the muscle coat of stomach of 3 hour POI groups compared with jejunum and ileum of POI groups or sham operated groups (p<0.05). There was strong association between the degree of inflammation and calprotectin positive cells in stomach.
CONCLUSION
Postoperative ileus induced by cecal manipulation significantly increased the degree of inflammation and calprotectin positive cells in the muscle coat of stomach as a remote organ. The relevance of degree of inflammation and the recovery time of ileus should be pursued in the future research.

Keyword

Postoperative ileus; stomach; small intestine; calprotectin

MeSH Terms

Animals
Charcoal
Guinea Pigs
Ileus/*immunology
Male
Muscle, Smooth/*immunology
Stomach/*immunology
Charcoal

Figure

• 

  Fig. 1 Distances of charcoal migration of POI and sham operation groups. The distance of charcoal migration was reduced significantly in the 3 hour POI groups (n=8) compared with sham operation groups (n=7) (7.25±1.76 cm vs. 99.07±16.78 cm). The distance of charcoal migration was reduced significantly in the 6 hour POI groups (n=8) compared with sham operation groups (n=8) (40.63±5.99 cm vs. 116.25±11.27 cm).

• 

  Fig. 2 Correlation between degree of inflammation and calprotectin positive cells.

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