Korean J Parasitol.  2014 Jun;52(3):325-329.

Phylogenetic Relationships of 3 Korean Neodiplostomum Species (Digenea: Neodiplostomidae) Based on Partial CO1 Gene

• Pyo KH
• Yi Lee JW
• Lee JJ
• Park YK
• Shin EH
• Chai JY

Abstract

The phylogenetic relationships of the 3 Neodiplostomum spp. (Digenea: Neodiplostomidae) occurring in Korea (N. seoulense, N. leei, and N. boryongense) were analyzed using the partial mitochondrial cytochrome c oxidase subunit 1 (CO1) gene. The adult flukes were recovered from Sprague-Dawley rats (N. seoulense) and newborn chicks (N. leei and N. boryongense) experimentally infected with the neodiplostomula from the grass snake, Rhabdophis tigrinus tigrinus. The genomic DNA was amplified using specific primers, and the sequence of CO1 was obtained. According to the results, the pairwise similarity was 96.1% between N. boryongense and N. seoulense, but was 95.0% between N. boryongense and N. leei and 94.2% between N. leei and N. seoulense. The results demonstrated a closer phylogenetic relationship between N. seoulense and N. boryongense. This high relationship of N. seoulense and N. boryongense may be related to their similar morphologic features including the limited distribution of vitellaria and the presence of a genital cone. N. leei is distinct on the other hand with an extensive distribution of vitellaria and the absence of a genital cone.

Keyword

Neodiplostomum seoulense; Neodiplostomum leei; Neodiplostomum boryongense; phylogeny; CO1 gene

MeSH Terms

Animals
Base Sequence
Chickens
Cluster Analysis
Colubridae/*parasitology
DNA, Helminth/chemistry/genetics
Electron Transport Complex IV/*genetics
Female
Korea
Molecular Sequence Data
Phylogeny
Rats, Sprague-Dawley
Sequence Analysis, DNA
Sequence Homology, Nucleic Acid
Trematoda/*classification/*genetics
DNA, Helminth
Electron Transport Complex IV