Korean J Intern Med.  2010 Mar;25(1):82-85. 10.3904/kjim.2010.25.1.82.

Detecting Bacterial Growth in Continuous Ambulatory Peritoneal Dialysis Effluent Using Two Culture Methods

• Yoon SH
• Choi NW
• Yun SR

• KMID: 1785659
• DOI: http://doi.org/10.3904/kjim.2010.25.1.82

Abstract

BACKGROUND/AIMS
The aim of this study was to evaluate the peritonitis-causing bacteria detected in peritoneal fluid using a blood culture bottle in patients undergoing continuous ambulatory peritoneal dialysis (CAPD).
METHODS
One-hundred and eleven dialysates from 43 patients suspected of peritonitis related to CAPD were retrospectively evaluated between May 2000 and February 2008. In all cases, 5 to 10 mL of dialysate was inoculated into a pair of BacT/Alert blood culture bottles, and 50 mL of centrifuged dialysate was simultaneously inoculated into a solid culture media for conventional culture. The results were compared to those of the conventional culture method. Isolated microorganisms were compared between the two methods.
RESULTS
The blood culture method was positive in 78.6% (88 / 112) of dialysate specimens and the conventional culture method in 50% (56 / 112, p < 0.001).
CONCLUSIONS
The blood culture method using the BacT/Alert system is useful for culturing dialysates and improves the positive culture rate in patients with suspected peritonitis compared to the conventional culture method.

Keyword

Culture media; Peritoneal dialysis; Peritonitis; Sensitivity and specificity

MeSH Terms

Culture Media
Dialysis Solutions
Gram-Negative Bacterial Infections/*diagnosis/microbiology
Gram-Positive Bacterial Infections/*diagnosis/microbiology
Humans
Kidney Failure, Chronic/*therapy
Microbiological Techniques/*methods
Peritoneal Dialysis, Continuous Ambulatory/*adverse effects
Peritonitis/*diagnosis/microbiology
Sensitivity and Specificity