J Korean Med Sci.  2005 Oct;20(5):888-891. 10.3346/jkms.2005.20.5.888.

Detection of Bartonella henselae DNA by Polymerase Chain Reaction in a Patient with Cat Scratch Disease: A Case Report

• Chung JY
• Han TH
• Kim BN
• Yoo YS
• Lim SJ

• KMID: 1781778
• DOI: http://doi.org/10.3346/jkms.2005.20.5.888

Abstract

We report a case of cat scratch disease caused by Bartonella henselae in Korea. A 25-yr-old woman developed left cervical lymphadenopathy with history of contact with a dog. The cervical lymphadenopathy persisted for 1 month and resolved gradually and spontaneously. Serologic test was not done during the acute stage of the disease. Immunofluorescent antibody test performed during the convalescent stage was positive for B. henselae. To confirm B. henselae infection, polymerase chain reaction (PCR) analysis using aspirates of cervical lymph node was performed and the presence of B. henselae DNA was demonstrated. This is the first reported case of cat scratch disease in Korea confirmed by PCR for B. henselae DNA.

Keyword

Bartonella henselae; Cat-Scratch Disease; Polymerase Chain Reaction

MeSH Terms

Adult
Bartonella henselae/*genetics/*isolation and purification
Cat-Scratch Disease/*diagnosis/*microbiology
DNA, Bacterial/*analysis/*genetics
Female
Humans
Polymerase Chain Reaction/*methods

Figure

• 

  Fig. 1 Results of seminested polymerase chain reaction (PCR) for gltA gene and pap 31 gene of B. henselae. Lanes 2-4 for PCR of gltA gene (139 bp), lane 6-8 for PCR of pap31 gene (211 bp). Lane 1 and 5, DNA ladder marker (Bioneer, Daejeon, Korea); lane 2 and 6 positive control (Houston-1, ATCC 49882); lane 3 and 7, negative control; lane 4 and 8, lymph node tissue from the patient with cat scratch disease.

• 

  Fig. 2 Partial pap31 sequences of two main genogroups B. henselae and case (red color-primer, blue color-different sequence between genogroups).

Reference

1. Anderson BE, Neuman MA. Bartonella spp. As emerging human pathogens. Clin Microbiol Rev. 1997. 10:203–219.

2. Carithers HA. Cat-scratch disease: an overview based on a study of 1,200 patients. Am J Dis Child. 1985. 139:1124–1133.

3. Regnery RL, Olson JG, Perkins BA, Bibb W. Serological response to "Rochalimaea henselae" antigen in suspected cat scratch disease. Lancet. 1992. 339:1443–1445.

4. Sander A, Posselt M, Bohm N, Ruess M, Altwegg M. Detection of Bartonella henselae DNA by two different PCR assays and determination of the genotypes of strains involved in histologically defined cat scratch disease. J Clin Microbiol. 1999. 37:993–997.

5. Margolis B, Kuzu I, Hermann M, Raible MD, Hsi E, Alkan S. Rapid polymerase chian reaction-based confirmation of cat scratch disease and Bartonella henselae infection. Arch Pathol Lab Med. 2003. 127:706–710.

6. Zeaiter Z, Fournier PE, Raoult D. Genomic variation of Bartonella henselae strains detected in lymph nodes of patients with cat scratch disease. J Clin Microbiol. 2002. 40:1023–1030.

7. Windsor JJ. Cat scratch disease: epidemiology, aetiology and treatment. Br J Biomed Sci. 2001. 58:101–110.

8. Murakami K, Tsukahara M, Tsuneoka H, Iino H, Ishida C, Tsujino K, Umeda A, Furuya T, Kawauchi S, Sasaki K. Cat scratch disease: analysis of 130 seropositive cases. J Infect Chemother. 2002. 8:349–352.

9. Jacobs RF, Schutze GE. Bartonella henselae as a cause of prolonged fever and fever of unknown origin in children. Clin Infect Dis. 1998. 26:80–84.

10. Chae MB, Lee JY, Kwak YG, Park SH, Lim HJ, Park SW, Chung MH, Kim MK, Kang JS. Prevalence of antibodies to Bartonella henselae and Bartonella quintana in Korean patients with lymphadenopathy. Korean J Infect Dis. 2002. 34:305–310.

11. Tsukahara M, Tsuneoka H, Iino H, Ohno K, Murano I. Bartonella henselae infection from a dog. Lancet. 1998. 352:1682.

12. Keret D, Giladi M, Kletter Y, Wientoub S. Cat scratch disease osteomyelitis from a dog scratch. J Bone Joint Surg Br. 1998. 80:766–767.

13. Sander A, Berner R, Ruess M. Serodiagnosis of cat scratch disease: response to Bartonella henselae in children and a review of diagnostic methods. Eur J Clin Microbiol Infect Dis. 2001. 20:392–401.

14. Avidor B, Varon M, Marmor S, Lifschitz-Mercer B, Kletter Y, Ephros M, Giladi M. DNA amplification for the diagnosis of cat scratch disease in small quantity clinical specimens. Am J Clin Pathol. 2001. 115:900–909.