Korean J Lab Med.  2010 Jun;30(3):284-288. 10.3343/kjlm.2010.30.3.284.

First Report of Brain Abscess Associated with Pseudozyma species in a Patient with Astrocytoma

• Hwang S
• Kim J
• Yoon S
• Cha Y
• Kim M
• Yong D
• Chang JH
• Jeong SH
• Uh Y
• Lee K

• KMID: 1781628
• DOI: http://doi.org/10.3343/kjlm.2010.30.3.284

Abstract

A yeast-like strain was isolated from the brain abscess of a patient diagnosed with astrocytoma. Morphological and molecular analysis on D1/D2 domain in the 26S rRNA gene and internal transcript spacer region of the strain revealed that the strain belonged to the genus Pseudozyma. To the best of our knowledge, this is the first report on the isolation of a Pseudozyma strain from brain abscess.

Keyword

Pseudozyma species; Molecular diagnostic techniques; Brain abscess

MeSH Terms

Aged
Astrocytoma/*complications
Brain Abscess/complications/diagnosis/*microbiology
Brain Diseases/*complications
DNA, Fungal/genetics
Humans
Male
Mycological Typing Techniques
Phylogeny
RNA, Ribosomal/genetics
Ustilaginales/classification/genetics/*isolation &purification

Figure

• 

  Fig. 1. (A) Colony morphology after 3 days of incubation on Sabouraud's dextrose agar at 30°C and (B) gram staining showing fusiform yeast with budding (Gram stain,×1,000). (C) Slide culture on Sabouroud's dextrose agar after 3 days of incubation at 30°C in ambient air showing elongated spindle-shaped blastoconidia. Scale bar=10 μm.

• 

  Fig. 2. Molecular phylogenetic tree of strain YCM09/6/F44 and other related reference species derived from the sequence of D1/D2 domain of 26S rRNA. The bar indicates genetic distance.

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