Korean J Lab Med.  2007 Aug;27(4):257-264. 10.3343/kjlm.2007.27.4.257.

Distribution of TT Virus Genotypes and Genogroups in 69 Healthy and 59 Hepatitis B Virus Infected Korean Individuals

• Kim HS
• Kim JS
• Park MJ
• Song W
• Kang HJ
• Lee KM

• KMID: 1781494
• DOI: http://doi.org/10.3343/kjlm.2007.27.4.257

Abstract

BACKGROUND: TT virus (TTV) infection is highly prevalent in general population and patients with hepatitis B virus (HBV) infection. The aim of the present study was to determine the distribution of the genotypes and genogroups of TTV in healthy and HBV-infected individuals in Korea. METHODS: Distribution of TTV genotypes and genogroups was investigated in the serum samples of 69 healthy and 59 HBV-infected individuals. PCR products of N22 region were genotyped by sequence analysis. TTV genogroups were determined by 5 different genogroup-specific PCR assays. RESULTS: Among the 20 sequenced isolates, 9 (45%) were genotype 2, 8 (40%) were genotype 1, 2 (10%) were genotype 3, and 1 (5%) was genotype 4. TTV genogroup 4 was found most frequently (52/128), followed by genogroup 3 (42/128), genogroup 1 (35/128), genogroup 5 (32/128), and genogroup 2 (1/128). Mixed infections with different genogroups were frequent. CONCLUSIONS: TTV genotype 2 and 1 are predominant genotypes. TTV genotype 3 was detected for the first time in Korea. TTV genogroups 4 and 3 were predominant genogroups. No significant difference was observed in the distribution of TTV genogroups between healthy and HBV-infected individuals.

Keyword

TT virus (TTV); Genotype; Genogroup

MeSH Terms

Adult
Amino Acid Sequence
DNA Virus Infections/diagnosis/*virology
Female
Genotype
Hepatitis B/*complications/diagnosis
Humans
Korea
Male
Middle Aged
Molecular Sequence Data
Phylogeny
Polymerase Chain Reaction/methods
Torque teno virus/classification/*genetics

Figure

• 

  Fig. 1. Phylogenetic tree generated by the neighbor-joining method of the nucleotide sequence of the N22 region (nt 1939–2142) of TTV isolates from 15 healthy and 5 HBV-infected individuals. H, healthy individuals; B, HBV-infected individuals; G1a, genotype 1a; G1b, genotype 1b; G2a, genotype 2a; G2b, genotype 2b; G2c, genotype 2c; G2d, genotype 2d; G3, genotype 3; G4, genotype 4; G5, genotype 5; AB008394, AB016942, AB017769, AB017879, AB017770, AB017771, AB016936, AB016952, AB018960, AB-017774, AB017775, AB017887, and AB017776 are the GenBank database-derived isolates.

• 

  Fig. 2. Deduced amino acid sequences of TTV genotype 1 (A) and TTV genotype 2 (B). Amino acid sequences are deduced from the nucleotide sequences of the N22 region (nt 1939–2142). Below the sequences, well-conserved and identical amino acid positions are indicated by colon and asterisk symbols, respectively. H, healthy individuals; B, HBV-infected individuals; AB008394, AB016942, AB017769, AB017879, AB017770, AB017771, AB-016952 and AB016936 are the GenBank database-derived isolates.

Reference

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