Ann Lab Med.  2014 Jan;34(1):71-75. 10.3343/alm.2014.34.1.71.

Necrotizing Pneumonia and Empyema in an Immunocompetent Patient Caused by Nocardia cyriacigeorgica and Identified by 16S rRNA and secA1 Sequencing

Affiliations
  • 1Department of Laboratory Medicine, Kangbuk Samsung Hospital, Sungkyunkwan University School of Medicine, Seoul, Korea. mjkkmd@gmail.com
  • 2Department of Laboratory Medicine and Genetics, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea.
  • 3Division of Infectious Diseases, Kangbuk Samsung Hospital, Sungkyunkwan University School of Medicine, Seoul, Korea.

Abstract

No abstract available.


MeSH Terms

Aged
Bacterial Proteins/genetics
Empyema/complications/diagnosis/*microbiology
Humans
Immunocompromised Host
Male
Nocardia/classification/*genetics/isolation & purification
Phylogeny
Pneumonia/complications/diagnosis/*microbiology
Positron-Emission Tomography
RNA, Ribosomal, 16S/analysis
Sequence Analysis, DNA
Tomography, X-Ray Computed
Bacterial Proteins
RNA, Ribosomal, 16S

Figure

  • Fig. 1 (A) Both chest radiograph (A) and computed tomography scan of the thorax (B) demonstrate bilateral pleural effusions.

  • Fig. 2 Colony morphology on sub-inoculation and microscopic morphology. (A) Chalky-white colonies on blood agar plates at 36℃ in 5% CO2. (B) Beaded, branching, gram-positive rods of N. cyriacigeorgica (modified acid-fast bacilli stain, ×400).

  • Fig. 3 Molecular phylogenetic tree constructed by the neighbor-joining method by using (A) 16s rRNA and (B) secA1 gene sequences from the N. cyriacigeorgica isolate and various Nocardia species. Reference sequences indicating the species strain and accession numbers are given in parentheses. All names and accession numbers are as cited in GenBank. Scale bar represents 5 nucleotide substitutions per 1,000 nucleotides.


Reference

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