J Korean Acad Periodontol.
1999 Jun;29(2):349-354. 10.5051/jkape.1999.29.2.349.
Development of PCR Technology for Identification of the Restriction Fragment Length Polymorphism(RFLP) of the Immunoglobulin Allotypes in Periodontal Patients
- Affiliations
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- 1Department of Periodontology, School of Dentistry, Pusan National University, Korea.
- 2Department of Biochemistry, School of Medicine, Dong-A University, Korea.
- KMID: 1739582
- DOI: http://doi.org/10.5051/jkape.1999.29.2.349
Abstract
- The present study has been performed to develop a PCR technology to identify human immunoglobulin(Ig) allotypes with restriction fragment length polymorphism(RFLP) using a probe. Genomic DNA were ampilified with PCR tecnology using primers from peripheral blood lymphocytes of 10 periodontal patiens, whose Ig allotypes have been pre-determined by serological tecnique using heagglutination technique. The result indicated that the RFLP patterns could successfully differentiate the Ig allotypes, which suggests that this technology can be developed as a tool useful for population genetics studies.
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