Yonsei Med J.  1985 Dec;26(2):175-183. 10.3349/ymj.1985.26.2.175.

Properties of Hepatitis B Virus Associated DNA Polymerase

• Oh SH
• Park YH

• KMID: 1729535
• DOI: http://doi.org/10.3349/ymj.1985.26.2.175

Abstract

The nature of hepatitis B virus (HBV) particle associated DNA polymerase was studied in relation to various enzyme inhibitors including antiviral agents. HBV DNA polymerase required high concentration of MgCl2(> 30 mM) and neutral pH for its full activity. p-chloromercuribenzoate was a strong inhibitor (85% inhibition at 1 mM) but N-ethylmaleimide had much less inhibitory effect (20% inhibition at 10 mM). Phosphonoformic acid showed the greatest inhibitory effect on HBV-DNA polymerase (almost complete inhibition at 100 microM) among phosphocompounds tested. Adenine arabinoside triphosphate (ara-ATP) and cytosine arabinoside triphosphate (ara-CTP) were competitive inhibitors with respect to their respective deoxyribonucleoside triphosphate (dATP and dCTP). Ara-CPT was a stronger inhibitor of HBV-DNA polymerase compared to ara-ATP. Ki values for ara-ATP and ara-CTP were 15.0 microM and 11.7 microM , respectively. HBV-DNA polymerase is characteristic in its ionic requirements and susceptibilities to certain inhibitors.

Keyword

HBV-DNA polymerase; PFA; ara-ATP; ara-CTP

MeSH Terms

DNA-Directed DNA Polymerase/antagonists & inhibitors
DNA-Directed DNA Polymerase/metabolism*
Hepatitis B Virus/enzymology*
Human