Korean J Parasitol.  2009 Jun;47(2):171-174. 10.3347/kjp.2009.47.2.171.

Ultrastructural Localization of Cryptosporidium parvum Antigen Using Human Patients Sera

Affiliations
  • 1Department of Environmental and Tropical Medicine, Konkuk University School of Medicine, Seoul 143-701, Korea. maria205@kku.ac.kr
  • 2Department of Parasitology, Kangwon National University College of Medicine, Chuncheon 200-701, Korea.
  • 3Department of Radiation Oncology, College of Medicine, Chungbuk National University, Cheongju 361-711, Korea.

Abstract

The antigen location of Cryptosporidium parvum, which stimulates antibody formation in humans and animals, was investigated using infected human sera. Immuno-electron microscopy revealed that antigenicity-inducing humoral immunity was located at various developmental stages of parasites, including asexual, sexual stages, and oocysts. The amount of antigen-stimulating IgG antibodies was particularly high on the oocyst wall. The sporozoite surface was shown to give stimulation on IgG and IgM antibody formation. Trophozoites implicated the lowest antigenicity to humoral immunity, both IgG and IgM, by showing the least amount of gold labeling. Immunogold labeling also provided clues that antigens were presented to the host-cell cytoplasm via feeder organelles and host-parasite junctions.

Keyword

Cryptosporidium parvum; cryptosporidiosis; immuno-electron microscopy; antigenicity; human serum
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