Korean J Parasitol.  2006 Sep;44(3):233-237. 10.3347/kjp.2006.44.3.233.

Cloning of the novel putative apoptosis-related gene of Spirometra erinacei (Order Pseudophyllidae)

• Lee SU
• Huh S

• KMID: 1720522
• DOI: http://doi.org/10.3347/kjp.2006.44.3.233

Abstract

We postulated that apolysis was processed in accordance with apoptotic changes occurring in a cestode, Spirometra erinacei (Pseudophyllidea). We cloned the novel putative apoptosis-associated gene from S. erinacei via screening of a S. erinacei cDNA library with a ced-3 gene (activator of apoptosis) probe from Caenorhabditis elegans. We identified a 261-bp cDNA sequence, which encodes for an 86-amino acid protein. The cloned gene expression was observed in the neck and gravid proglottids via Northern blotting, using cloned cDNA inserts as probes, but the clone was not expressed in any of other tissues. We suggest that this gene may be involved in the apolysis of S. erinacei during normal tissue development and differentiation in cestode parasites.

Keyword

Spirometra erinacei; cestoda; apolysis; apoptosis; cDNA library

MeSH Terms

Spirometra/*genetics/growth & development/physiology
Molecular Sequence Data
Gene Library
Cloning, Molecular
Caspases/genetics
Caenorhabditis elegans Proteins/genetics
Base Sequence
Apoptosis/*genetics
Animals
Amino Acid Sequence