Korean J Parasitol.  2011 Dec;49(4):381-384. 10.3347/kjp.2011.49.4.381.

Molecular Identification of a Trichinella Isolate from a Naturally Infected Pig in Tibet, China

• Li LZ
• Wang ZQ
• Jiang P
• Zhang X
• Ren HJ
• Cui J

• KMID: 1715743
• DOI: http://doi.org/10.3347/kjp.2011.49.4.381

Abstract

The first human case with trichinellosis was reported in 1964 in Tibet, China. However, up to the present, the etiological agent of trichinellosis has been unclear. The aim of this study was to identify a Tibet Trichinella isolate at a species level by PCR-based methods. Multiplex PCR revealed amplicon of the expected size (173 bp) for Trichinella spiralis in assays containing larval DNA from Tibet Trichinella isolate from a naturally infected pig. The Tibet Trichinella isolate was also identified by PCR amplification of the 5S ribosomal DNA intergenic spacer region (5S ISR) and mitochondrial large-subunit ribosomal RNA (mt-lsrDNA) gene sequences. The results showed that 2 DNA fragments (749 bp and 445 bp) of the Tibet Trichinella isolate were identical to that of the reference isolates of T. spiralis. The Tibet Trichinella isolate might be classifiable to T. spiralis. This is the first report on T. spiralis in southwestern China.

Keyword

Trichinella spiralis; taxonomy; molecular identification; Tibet; China

MeSH Terms

Animals
DNA, Helminth/chemistry/genetics
DNA, Mitochondrial/chemistry/genetics
DNA, Ribosomal/chemistry/genetics
DNA, Ribosomal Spacer/genetics
Genotype
Humans
Multiplex Polymerase Chain Reaction
RNA, Ribosomal, 5S/genetics
Sequence Analysis, DNA
Swine
Swine Diseases/*parasitology
Tibet
Trichinella spiralis/*classification/genetics/isolation & purification
Trichinellosis/parasitology/*veterinary
DNA, Helminth
DNA, Mitochondrial
DNA, Ribosomal
DNA, Ribosomal Spacer
RNA, Ribosomal, 5S