Korean J Parasitol.  1993 Sep;31(3):215-222. 10.3347/kjp.1993.31.3.215.

Comparative susceptibility of different cell lines for culture of Toxoplasma gondii in vitro

• Park BK
• Moon HR
• Yu JR
• Kook J
• Chai JY
• Lee SH

• KMID: 1715465
• DOI: http://doi.org/10.3347/kjp.1993.31.3.215

Abstract

In order to establish a useful cell culture system for T. gondii, we compared the degree of proliferation of T. gondii tachyzoites among 8 different cell lines; 2 kinds of normal animal cells (MDCK-canine kidney cells; Vero-monkey kidney cells) and 6 kinds of human tumor cells (A 549, PC 14-lung cancer cells; SNU 1, SNU 16, MKN 45-stomach cancer cells; HL-60-promyelocytic leukemia cells), through morphological observation and 3H-uracil uptake assay. The degree of susceptibility to infection with T. gondii tachyzoites was highest in A 549 and PC 14 cells, medium in Vero, HL-60, MDCK and SNU 1, and lowest in SNU 16 and MKN 45 cells. The kinetics of T. gondii multiplication during the post-infection 60 hours were highly dependent upon the dose of tachyzoites administered and the duration of cultivation. These results show that A 549 and PC 14 are the most suitable cell lines among the 8 tested for the growth and multiplication of T. gondii in vitro.

MeSH Terms

Cell-Line-parasitology
Dogs-
English-Abstract
Haplorhini-
Time-Factors
Tumor-Cells,-Cultured-parasitology
*Toxoplasma-growth-and-development