Korean J Parasitol.
1994 Jun;32(2):111-116. 10.3347/kjp.1994.32.2.111.
Immunogenicity and protective efficacy of solubilized merozoite-enriched Theileria sergenti immmunogens III. Characterization of immunodominant peptides
- Affiliations
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- 1Chonbuk National University, College of Veterinary Medicine, chonju 560-756, Korea.
- 2Veterinary Research Institute, Ahnyang 430-016, Korea.
- 3University of Illinois, Urbana IL 61801 USA.
- KMID: 1715448
- DOI: http://doi.org/10.3347/kjp.1994.32.2.111
Abstract
- Immunoblot analysis utilizing bovine sera from naturally or experimentally infected with Theileria sergenti were used to determine the immunodominant polypeptides of T. sergenti (Korea isolate).The previously recognized major bands, 18 kDa, 29 kDa, 34 kDa, and 45 kDa, were excised after electrophoresis and trasferred to PVDF membrane. The individual bands were sequenced. The 34 kDa polypeptide which was the most antigenic and immunogenic peptide was observed in the Western blot. However, Chou-Fasman prediction sites (antiginic site) for antigen determinants of the 45 kDa,34 kDa, 29 kDa and 18 kDa polypeptide were 6, 4, 2 and 0, respectively. However, the 45 kDa polypeptide showed no reaction with anti-T. sergenti hyperimmune serum.
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