Korean J Parasitol.  1984 Jun;22(1):1-10. 10.3347/kjp.1984.22.1.1.

Study on Metagonimus yokogawai(Katsurada, 1912) in Korea VII. Electron microscopic observation on the tegumental structure

• Lee SH
• Seo BS
• Chai JY
• Hong SJ

• KMID: 1715113
• DOI: http://doi.org/10.3347/kjp.1984.22.1.1

Abstract

This study was performed to observe the chronological changes in the tegumental structure of M. yokogawai using scanning electron microscope. The subjected worms were excysted metacercariae obtained from sweetfish, and 2-day, 1-week and 4-week old worms experimentally reared in albino rats. The results are as follows: The tegument of excysted metacercariae showed many transverse shallow rugae, which were more remarkable in posterior half body, i.e., posterior to ventral sucker. The whole surface was armed with many scale-like spines; 7-8 pointed ones on anterior body and 2-3 pointed on posterior body. The ciliated knob-like papillae (Type I) were abundant around oral and ventral suckers, which grouped 2, 3 or 4 in number in most cases. A few round swellings of tegument (Type II) were observed only on oral sucker. The tegumental surface of 2-day old worms showed deeper rugae, and the anterior half covered with knob-like processes of distal cytoplasm and the posterior half with cobblestone-like ones. Interspinous space became more wide and 9 pointed spines appeared on anterior dorsal surface. The sensory papillae enlarged but not changed in their distribution. The tegument of 1-week old worms revealed knob-like cytoplasmic processes in posterior half body and velvety ones around oral sucker. The scale-Iike spines of anterior half body changed remarkably to the slender ones of posterior body at the level of ventral sucker. In dorsal surface, the arrangement of the Type I papillae were bilaterally symmetrical. The tegument of 4-week old worms were finely differentiated and the posterior tegument covered with velvety cytoplasmic processes. The spines had remarkably grown in length and width but the density remained nearly unchanged. The papillae also became larger but their morphology and distribution were not different from younger worms. However, the round elevation of cytoplasmic ridges (Type III papilla) appeared bilaterally on inner wall of oral sucker, approximately 8 in number. From the above results, it is considered that the tegument of juvenile M. yokogawai continued to differentiate until 4 weeks after infection.

MeSH Terms

parasitology-helminth-trematoda
Metagonimus yokogawai
electronmicroscopy
Plecoglossus altivelis
tegument