The Nitroblue-tetrazlium dye Reduction Test in Urinary Tract Infections

Lee, Sang Ju; Yoon, Jong Byung

Abstract

This present study was performed to evaluate the clinical usefulness of the NBT test and to take the information of self defense mechanism of neutrophil in 40 cases with normal group and 110 cases with various infection groups in urology, such as acute pyelonephritis, chronic pyelonephritis, neurogenic bladder with chronic pyuria and chronic prostatitis. The results were as followings: 1. In normal group, the mean +/- 2 S.D. of WBC/mm3 was 5,725 +/- 1,164, neutrophils/mm3 3,202 +/- 929 and % of NBT positive neutrophils 8.14 +/- 3. 99. 2. Infection group; In acute pyelonephritis, the mean +/- 2 S.D. of WBC/mm3 was 10, 110 +/- 3,155, neutrophil/mma 7,281 +/- 1,469 and % of NBT positive neutrophils 13.14 +/- 5.09. In chronic pyelonephritis, the mean +/- 2 S.D. of WBC/mm3 was 6.053 +/- 2,976, neutrophils/mm3 3,495 +/- 1,642 and % of NBT positive neutrophils 8.66 +/- 3.26. In neurogenic bladder with chronic pyuria, the mean +/- 2 S.D. of WEC/mm3 was 6,503 +/- 2,003, neutrophils/mm3 3,735 +/- 1,049 and % of NBT positive neutrophils 5.83 +/- 4.72. In chronic prostatitis, the mean +/- 2 S.D. of WEC/mm3 was 6,096 +/- 2,771, neutrophils/mm3 3,524 +/- 1,977 and % of NBT positive neutrophils 6.82 +/- 5.54. 3. WBC, neutrophils/mm3 and % of NBT positive neutrophils were significantly increased in acute pyelonephritis. 4. WBC, neutrophils/mm3 and % of NBT positive neutrophils were not significant in chronic pyelonephritis. 5. WBC, neutrophils/mm3 were not significant, but % of NBT positive neutrophils was significantly decreased in neurogenic bladder with chronic pyuria and chronic prostatitis. 6. Authors can conclude that the function of self defense mechanism of neutrophils enhanced in acute stage of infection, but weakened as infection continues.