J Korean Assoc Maxillofac Plast Reconstr Surg.  2004 Mar;26(2):151-154.

INFLUENCE OF DIFFERENT TYPES AND CONCENTRATIONS ON THE SURVIVAL OF RAPIDLY FROZEN OSTEOBLAST-LIKE CELLS: A PRELIMINARY STUDY

• Oh JH
• Lee BS
• Ryu DM
• Kim YG
• Kuebler A

Abstract

The aim of this study is to establish optimal conditions for obtaining high survival rate of osteoblast-like Human osteoblast-like cells isolated by serial enzymatic technique were exposed to variable cryoprotectants for 2 minutes in room temperature and then rapidly frozen with -196 degrees C nitrogen. Different concentrations of Me2SO, Ethylene glycol, and Sucrose were used for preventing cell injury during freezing and thawing. The cryoprotectants were designated 20% Ethylene glycol (EG), 40% EG, 40% EG containing 0.25 M Sucrose, 2.0 M Me2SO, 3.5 M Me2SO, and 3.5 M Me2SO containing 0.25 M Sucrose. Hemocytometer was used to calculate the survival rate and WST-1 was assessed for cell proliferation and The EG groups showed better survival rate (64.9%) and WST-1 (1.77) than those, 52.5% and 1.56, respectively, of Me2SO groups. The Combination of EG and Sucrose increase the survival rate of osteoblast-like cells. 40% EG containing 0.25 Sucrose group showed the best survival rate (70.1%) and The results of this study suggest that the ultrarapid freezing technique require high concentration of cryoprotectant and using of 40% EG with 0.25 M Sucrose decrease the cell injury during rapid freezing of human osteoblast-like cells.

Keyword

Ultrarapid Freezing Technique; Cryoprotectant; Osteoblast-like Cells

MeSH Terms

Cell Proliferation
Ethylene Glycol
Freezing
Humans
Nitrogen
Sucrose
Survival Rate
Ethylene Glycol
Nitrogen
Sucrose