J Korean Rheum Assoc.  2005 Jun;12(2):83-89.

MPO Gene Polymorphism in Behcet's Disease

• Cho JH
• Kim SU
• Seo JS
• Kang BS
• Nam EJ
• Kang YM

Abstract

OBJECTIVE
Behcet's disease (BD) is a systemic vasculitis in which hyperfunction of neutrophils is one of the major pathophysiologic features. Myeloperoxidase (MPO) is an important neutrophilic lysosomal enzyme and has been implicated in tissue damage of BD. As the A allele of -463G/A polymorphism of the MPO gene is associated with diminished activity of MPO, we analyzed the association of -463G/A polymorphism of the MPO gene with BD.
METHODS
We analyzed -463G/A polymorphism of the MPO gene in BD patients (n=101) and controls (n=138). Genotype of the MPO gene at position -463 was determined by PCR-RFLP assay using genomic DNA.
RESULTS
The allele frequency of -463G/A polymorphism of the MPO gene did not deviate from the Hardy-Weinberg expectation in both BD patients and controls. There were no statistically significant differences in genotype distribution and allele frequency between patients and controls at position -463 (p=0.761 and p=0.549 respectively). Analysis of genotype distribution and allele frequency of the -463G/A according to sex did not show any difference between patients and controls. There were no statistically significant differences in clinical manifestations of BD among different genotypes.
CONCLUSION
The present data indicate that the -463G/A polymorphism of the MPO gene was not associated with the susceptibility to and clinical manifestations of BD in Korea.

Keyword

Myeloperoxidase; Behcet's disease; Single nucleotide polymorphism

MeSH Terms

Alleles
DNA
Gene Frequency
Genotype
Humans
Korea
Neutrophils
Peroxidase
Polymorphism, Single Nucleotide
Systemic Vasculitis
DNA
Peroxidase