Korean J Anat.
1999 Jun;32(3):293-304.
Observation of Endothelial Cell Differentiation by 3D Cell culture system: Angiogenesis inhibition of Thrombospondin-1
- Affiliations
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- 1Department of Anatomy, College of Medicine, Chungbuk National University, Korea.
- 2Department of Anatomy, college of Medicine, Seoul National University, Korea.
- 3Department of Biochemistry and Biophysics, School of Medicine, Washington University, Korea.
Abstract
- Angiogenesis plays a fundamental role in development of circulation system, reorganization of reproductive system, wound healing. Pathological angiogenesis is deeply involved in a variety of diseases, particularly solid tumor growth and metastasis. However, it is not easy to study the mechanism of angiogenesis because endothelial cells proceed complex differentiation by interaction with extracellular matrix proteins and growth factors. However, human umbilical vein endothelial cells (HUVEC) form polygonal networks of capillary-like tubes in 3D Matrigel cultures. Differentiation of endothelial cells will be observed accurately by application of videomicroscopy. Thrombospondin-1 is secreted by a wide variety of cells including endothelial cells and is incorporated into their matrix. Thrombospondin-1 can modulate differentiation of endothelial cells by increasing cell-cell interactions as well as cell-substrate interactions. The current study was undertaken to determine which mechanism is involved in inhibition of angiogenesis by Thrombospondin-1. They was secreted from HUVEC during the process of angiogenesis in 3D Matrigel culture. When applied to endothelial cells attachment to the surface of Matrigel was not decreased, but spreading was decreased. In addition, bigger clusters was formed by enhancement of cell to cell binding by Thrombospondin-1. They inhibit cord and tube formation of HUVEC by inhibition of migration. These results suggest that Thrombospondin-1 inhibits angiogenesis by blocking differentiation of endothelial cells to motile phenotype in 3D Matrigel culture.
MeSH Terms
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Cell Culture Techniques*
Endothelial Cells*
Extracellular Matrix Proteins
Human Umbilical Vein Endothelial Cells
Intercellular Signaling Peptides and Proteins
Microscopy, Video
Neoplasm Metastasis
Neovascularization, Pathologic
Phenotype
Wound Healing
Extracellular Matrix Proteins
Intercellular Signaling Peptides and Proteins
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