The effect of preosteoblast graft on osseous regeneration of the cranial bone defect in mouse

Park, Hong Ju

Abstract

The present study was performed to examine the effect of preosteoblast graft on bone defect of the mouse calvaria after culture. MC3T3-E1(MC3T3) cells were grafted after culture in the abscence of ascorbic acid (AA) and beta-glycerophosphate (GP) (group 1) or culture with AA and GP for differentiation of MC3T3 cells and formation of mineralized nodule (group 2). Twenty days after treatment with AA and GP, the mineralized nodule were formed in group 2. The mineralized nodule was not formed in the culture flask without AA and GP until 3 weeks in group 1. In control group, there was no regeneration on the defect at week 4. In experimental groups, inflammatory cell infiltration in the periphery of grafted cell pellet was noted at 1 week. The grafted cell pellet was well remained in the bony defect at weeks 1 and 2. In experimental group I, the newly formed woven bone was observed at week 2. In experimental group II, woven bone from the grafted cell pellet was observed at week 1, and more newly formed woven bone was observed at week 2. At week 4, generalized cancellous bone formation in the defect was observed in group 1, whereas cancellous bone and bone marrow was formed in experimental group II. These results demonstrate that the cultured preosteoblasts have the ability of new bone formation and differentiated osteoblasts by AA and GP are more effective on bone regeneration than preosteoblasts.