J Korean Acad Periodontol.  2008 Mar;38(1):83-90. 10.5051/jkape.2008.38.1.83.

Histomorphometric evaluation of bone healing with natural calcium carbonatederived bone substitutes in rat calvarial defect

• Lee CH
• Jang JH
• Lee JM
• Suh JY
• Park JW

• KMID: 1486788
• DOI: http://doi.org/10.5051/jkape.2008.38.1.83

Abstract

PURPOSE
This study investigated the osteoconductivity of natural calcium carbonate-derived bone substitutes, hen eggshell (ES), and compared with those of commercial bone substitutes.
MATERIALS AND METHODS
Osseous defects created in the rat calvaria were filled with particulated ES(ES-1), ES with calcium-deficient hydroxyapatite surface layer (ES-2), Biocoral(Inoteb, France), and Bio-Oss(Geistlich Pharma, Wolhusen, Switzerland). After 4 and 8 weeks of healing, histomorphometic analysis was performed to evaluate the amount of newly formed mineralized bone area (NB%).
RESULTS
Histologic and histomorphometric analysis showed new bone formation and direct bony contact with the grafted materials in all groups. At 4 weeks, Biocoral group showed greater NB% compared to Bio-Oss and ES-1 groups (P<0.05). At 8 weeks, Biocoral and ES-2 groups showed significantly greater NB% compared to Bio-Oss group (P<0.05).
CONCLUSION
These results indicate that natural calcium carbonate-derived bone substitutes with microporous calciumdeficient hydroxyapatite surface layer may be an effective materials treating osseous defects.

Keyword

bone substitute; calcium carbonate; osteoconductivity; histomorphometry

MeSH Terms

Animals
Bone Substitutes
Calcium
Calcium Carbonate
Durapatite
Minerals
Osteogenesis
Rats
Skull
Transplants
Bone Substitutes
Calcium
Calcium Carbonate
Durapatite
Minerals