J Korean Endocr Soc.  2008 Oct;23(5):319-326. 10.3803/jkes.2008.23.5.319.

Calpain-10 Polymorphism in Korean Women with Polycystic Ovary Syndrome

Affiliations
  • 1Department of Internal Medicine, Ewha Womans University School of Medicine, Korea.
  • 2Department of Obstetrics and Gynecology, Ewha Womans University School of Medicine, Korea.

Abstract

BACKGROUND: Polycystic ovary syndrome (PCOS) is characterized by chronic anovulation, hyperandrogenism and insulin resistance, and PCOS is known to be associated with an increased risk of type 2 diabetes mellitus (DM). PCOS has also been proposed to share a common genetic background with type 2 DM. The calpain 10 (CAPN10) gene is known to be associated with type 2 DM in several different population. We examined the association of CAPN10 gene polymorphisms and their influence on the metabolic abnormalities in Korean women who suffer with PCOS.
METHODS
One hundred sixty four women with PCOS and 325 control women were studied. The CAPN10 gene polymorphisms were genotyped by amplifying the genomic DNA. Anthropometric measures, a 75g oral glucose tolerance test and measurement of insulin sensitivity by the euglycemic hyperinsulinemic clamp technique were performed.
RESULTS
The frequencies of CAPN10 UCSNP-43, UCSNP-19, UCSNP-63 and the haplotype combinations were not different between the women with PCOS and the control subjects. In the women with PCOS and who had the UCSNP-43 GA genotype, the post-load 90 minute plasma glucose level was significantly greater and the HDL cholesterol and insulin mediated glucose uptake were significantly lower compared to the women with PCOS and who had the GG genotype.
CONCLUSION
The CAPN10 UCSNP-43 genotype might be responsible for insulin resistance, yet further study is required to confirm the role of this genetic polymorphism in the development of PCOS and the presentation of its disease features.

Keyword

calpain 10; insulin resistance; polycystic ovary syndrome

MeSH Terms

Anovulation
Calpain
Cholesterol, HDL
Diabetes Mellitus, Type 2
DNA
Female
Genotype
Glucose
Glucose Tolerance Test
Haplotypes
Humans
Hyperandrogenism
Insulin
Insulin Resistance
Plasma
Polycystic Ovary Syndrome
Polymorphism, Genetic
Calpain
Cholesterol, HDL
DNA
Glucose
Insulin

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