Korean J Thorac Cardiovasc Surg.
2012 Dec;45(6):368-379. 10.5090/kjtcs.2012.45.6.368.
Histologic Characteristics and Mechanical Properties of Bovine Pericardium Treated with Decellularization and alpha-Galactosidase: A Comparative Study
- Affiliations
-
- 1Department of Thoracic and Cardiovascular Surgery, Seoul National University College of Medicine, Korea.
- 2Department of Thoracic and Cardiovascular Surgery, Seoul National University Hospital, Seoul National University College of Medicine, Korea. kyj@plaza.snu.ac.kr
- KMID: 1433610
- DOI: http://doi.org/10.5090/kjtcs.2012.45.6.368
Abstract
- BACKGROUND
Bioprostheses for cardiovascular surgery have limitations in their use following as calicification. alpha-galactosidase epitope is known as a stimulant of immune response and then shows a progressing calcification. The objective of this study was to evaluate histologic characteristics and mechanical properties of decellularization and treated with alpha-galactosidase.
MATERIALS AND METHODS
Bovine pericardial tissues were allocated into three groups: fixation only with glutaraldehyde, decellularization with sodium dodesyl sulfate and decellularization plus treatment with alpha-galactosidase. We confirmed immunohistological characteristics and mechanical properties as fatigue test, permeability test, compliance test, tensile strength (strain) test and thermal stability test.
RESULTS
Decellularization and elimination of alpha-gal were confirmed through immunohistologic findings. Decellularization had decreased mechanical properties compared to fixation only group in permeability (before fatigue test p=0.02, after fatigue test p=0.034), compliance (after fatigue test p=0.041), and tensile strength test (p=0.00). The group of decellularization plus treatment with alpha-galactosidase had less desirable mechanical properties than the group of decellularization in concerns of permeability (before fatigue test p=0.043) and strain test (p=0.001).
CONCLUSION
Favorable decellularization and elimination of alpha-gal were obtained in this study through immunohistologic findings. However, those treatment including decellularization and elimination of alpha-gal implied the decreased mechanical properties in specific ways. We need more study to complete appropriate ioprosthesis with decellularization and elimination of alpha-gal including favorable mechanical properties too.
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