Nucl Med Mol Imaging.  2012 Mar;46(1):20-26.

Development of a 68Ga-Fluorinated Porphyrin Complex as a Possible PET Imaging Agent

• Fazaeli Y
• Jalilian A
• Amini MM
• Ardaneh K
• Rahiminejad A
• Bolourinovin F
• Moradkhani S
• Majdabadi

Abstract

AIM: Due to the interesting pharmacologic properties of porphyrins, the idea of developing a possible tumor imaging agent using PET by incorporating 68Ga into a suitable porphyrin ligand was investigated.
METHODS
68Ga-labeled 5,10,15,20-tetrakis(pentafluoro-13 phenyl) porphyrin (68Ga-TFPP) was prepared using freshly eluted [68Ga]GaCl3 obtained from a 68Ge/68Ga generator developed in-house and 5,10,15,20-tetrakis(pentafluoro-phenyl) porphyrin (H2TFPP) for 60 min at 100degrees C.
RESULTS
The complex was prepared with high radiochemical purity (>99% ITLC, >99% HPLC, specific degrees Cactivity: 13-14 GBq/mmol). Stability of the complex was checked in the final formulation and in human serum for 5 h. The partition coefficient was calculated for the compound (log P = 0.62). The biodistribution of the labeled compound in vital organs of Swiss mice bearing fibrosarcoma tumors was studied using scarification studies and SPECT imaging up to 1 h. The complex was mostly washed out from the circulation through kidneys and liver. The tumor-to-muscle ratio 1 h post injection was 5.13.
CONCLUSION
The radiolabeled porphyrin complex demonstrated potential for further imaging studies in other tumor models.

Keyword

Porphyrins; Ga-68; Fibrosarcoma; Biodistribution; Co-incidence

MeSH Terms

Animals
Chromatography, High Pressure Liquid
Fibrosarcoma
Humans
Kidney
Liver
Mice
Porphyrins
Tomography, Emission-Computed, Single-Photon
Ursidae
Porphyrins