Ann Lab Med.  2012 Sep;32(5):370-374. 10.3343/alm.2012.32.5.370.

First Korean Case of Robinsoniella peoriensis Bacteremia in a Patient with Aspiration Pneumonia

• Jeon Y
• Kim TS
• Kim HB
• Park KU
• Song J
• Kim EC

• KMID: 1387354
• DOI: http://doi.org/10.3343/alm.2012.32.5.370

Abstract

Robinsoniella peoriensis has recently been identified as a Gram-positive, spore-forming, anaerobic rod originally recovered from swine manure storage pits. To date, 6 cases of R. peoriensis infection have been reported, including 2 cases of bacteremia, 1 of abdominal fluid collection, and 3 of wound infection. In the present study, we report a 76-yr-old man with R. peoriensis bacteremia who developed aspiration pneumonia. Gram staining of a purified colony revealed Gram-positive, rod-shaped bacteria. Biochemical identification using API 20 A (bioMerieux, France) indicated presence of Clostridium spp. We performed both 500-bp and full-gene sequencing of 16S rRNA of the isolate. The sequence was analyzed with MicroSeq ID 16S rRNA Library v2.0 (Applied Biosystems, USA), GenBank Basic Local Alignment Search Tool (BLAST) (http://www.ncbi.nlm.nih.gov/genbank), and EzTaxon database v2.1 (http://www.eztaxon.org). The 500-bp 16S rRNA sequence of the blood culture isolate showed 99.16-99.79% similarity with R. peoriensis and the full-gene 16S rRNA sequence showed 98.87-99.50% similarity with R. peoriensis. The organism was confirmed as R. peoriensis by using all of the mentioned databases except for MicroSeq, which did not include the RNA sequence of this bacterium. This case suggests that identification of R. peoriensis might be challenging in clinical laboratories with no access to molecular methods, as certain commercial identification systems may not identify, or may misidentify, this organism. To the best of our knowledge, this is the first report of the isolation of R. peoriensis in Korea.

Keyword

Robinsoniella peoriensis; Bacteremia; 16S ribosomal RNA

MeSH Terms

Aged
Bacteremia/*microbiology
Clostridium/classification/genetics/*isolation & purification
Databases, Genetic
Humans
Male
Phylogeny
Pneumonia, Aspiration/*diagnosis/microbiology
RNA, Ribosomal, 16S/chemistry/genetics
Republic of Korea
Sequence Analysis, DNA

Figure

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  Fig. 1 Colonial and microscopic morphology of Robinsoniella peoriensis. (A) Dull white and flat colonies with mixed sizes and smooth edges on an anaerobic blood plate. (B) Gram-positive and rod-shaped bacteria with ovoid-shaped spores located sub-terminally (Gram staining; ×1,000).

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  Fig. 2 Unrooted neighbor-joining phylogenetic tree based on 16S rRNA sequences of Robinsoniella peoriensis and 31 similar organisms. Bootstrap values are provided at branching points. The scale bar represents 1 nucleotide substitution per 100 nucleotides.

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