Ann Lab Med.  2012 Sep;32(5):339-344. 10.3343/alm.2012.32.5.339.

Changes in the Levels of Interleukins 6, 8, and 10, Tumor Necrosis Factor Alpha, and Granulocyte-colony Stimulating Factor in Korean Burn Patients: Relation to Burn Size and Postburn Time

• Kim HS
• Kim JH
• Yim H
• Kim D

• KMID: 1387348
• DOI: http://doi.org/10.3343/alm.2012.32.5.339

Abstract

BACKGROUND
Major burn injury induces an inflammatory response that is accompanied by the release of various cytokines. We investigated the gradual changes in the levels of pro-inflammatory and anti-inflammatory cytokines following burn injury and determined the relationship between these levels and burn size in adult Korean patients with burn injury.
METHODS
Blood samples from 9 healthy controls and 60 Korean burn patients were collected on days 1, 3, 7, 14, and 21 after burn injury, and concentrations of interleukin (IL)-6, IL-8, IL-10, tumor necrosis factor (TNF)-alpha, and granulocyte-colony stimulating factor (G-CSF) were measured. Burn patients were divided into 3 groups according to burn size (15-30%, 31-50%, >50% total body surface area), and the concentrations of the cytokines were compared between these groups and the control group over 3 weeks.
RESULTS
Compared to their levels in controls, IL-6, IL-8, IL-10, TNF-alpha, and G-CSF levels in burn patients were significantly higher during the observation period. Median concentrations of IL-8, IL-10, and G-CSF at each time point increased with burn size, although peak levels and time to peak levels of these cytokines differed from patient to patient.
CONCLUSIONS
These findings indicate that IL-6, IL-8, IL-10, TNF-alpha, and G-CSF are important mediators in inflammatory changes after burn injury; however, various factors, including burn size, may influence the concentrations of these cytokines.

Keyword

Burns; Cytokines; Body surface area; Time

MeSH Terms

Adolescent
Adult
Aged
Asian Continental Ancestry Group
Burns/blood/*pathology
Granulocyte Colony-Stimulating Factor/*blood
Humans
Interleukin-10/*blood
Interleukin-6/*blood
Interleukin-8/*blood
Male
Middle Aged
Republic of Korea
Time Factors
Tumor Necrosis Factor-alpha/*blood
Young Adult

Figure

• 

  Fig. 1 IL-6 (A), IL-8 (B), IL-10 (C), TNF-α (D), and G-CSF (E) concentrations according to postburn time and burn sizes. Data are presented as median ± interquartile range. Solid lines and • indicate medians of all 3 groups at each time point.*Significant difference between Group I and Group II; †significant difference between Group II and Group III; ‡significant difference between Group I and Group III at each time point by Kruskal-Wallis test and post-hoc analysis.Abbreviations: IL, interleukin; TNF, tumor necrosis factor; G-CSF, granulocyte-colony stimulating factor.

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