Yonsei Med J.  2002 Aug;43(4):427-434. 10.3349/ymj.2002.43.4.427.

Altered Expression of Lewis Antigen on Tissue and Erythrocytes in Gastric Cancer Patients

Affiliations
  • 1Medical Research Office, Dongbu Korean Red Cross Blood Center.
  • 2Department of Laboratory Medicine, Yonsei University College of Medicine, Seoul, Korea. hyunok1019@yumc.yonsei.ac.kr
  • 3Department of Surgery, Yonsei University College of Medicine, Seoul, Korea.
  • 4Department of Pathology, Yonsei University College of Medicine, Seoul, Korea.
  • 5Yonsei Proteome Research Center, Yonsei University, Seoul, Korea.

Abstract

To elucidate the clinical significance of phenotypic alterations of Lewis antigen in gastric cancer patients, we investigated Lewis antigens by analyzing the genotypes of the Le and Se genes and by comparing the results obtained with the phenotypic expression of Lewis antigen in gastric cancer tissue and blood cells. One hundred and twenty gastric cancer patients were examined and compared with respect to Lewis blood phenotype and genotype. The expression of Lea, Leb, sialylated Lea, and sialylated Lex antigens was immunohistochemically examined in uninvolved gastric mucosa, intestinal metaplasia, and cancerous tissue. We also analyzed the significance of Lewis antigen expression by analyzing patient survival. The frequencies of the Lewis phenotypes of RBCs corresponding to Le(a+b-), Le(a-b+), and Le(a-b-) were 16%, 58%, and 26%, respectively. The Le and le allele gene frequencies calculated from genotyping in gastric cancer patients were 0.623 and 0.377, respectively. The frequency for Le(a-b-) of the RBC phenotype had a tendency to be higher in cancer patients than in normal healthy Koreans. However, no difference in the Lewis gene frequency was found between these gastric cancer patients and healthy persons. The phenotype of Le(a-b+) was most prevalent in uninvolved gastric mucosal tissue, whereas the most prevalent form in tumor tissue was Le(a-b-). Sialyl-Lea and sialyl-Lex antigens were hardly detectable in uninvolved gastric mucosa, whereas the two antigens were expressed highly in intestinal metaplastic mucosa and tumor cells. In conclusion, the loss of Lewis antigen expression in tissue and on RBCs in gastric cancer patients is not a result of genetic influences, but rather a result of sialylation in tissue. We also confirm that poor prognosis is associated with dimeric sialyl-Lex and vascular spread.

Keyword

Lewis antigen; Lewis genotype; gastric cancer; sialylation

MeSH Terms

Adult
Aged
Alleles
Erythrocytes/*chemistry
Female
Fucosyltransferases/*analysis/genetics
Gangliosides/analysis
Genotype
Human
Immunohistochemistry
Male
Metaplasia
Middle Age
Oligosaccharides/analysis
Phenotype
Stomach Neoplasms/*blood/genetics/mortality

Cited by  1 articles

The Relationship between Lewis/Secretor Genotypes and Serum Carbohydrate Antigen 19-9 Levels in a Korean Population
Hyung-Doo Park, Kyoung Un Park, Junghan Song, Chang-Seok Ki, Kyou Sup Han, Jin Q Kim
Korean J Lab Med. 2010;30(1):51-57.    doi: 10.3343/kjlm.2010.30.1.51.

Full Text Links
  • YMJ
Actions
Cited
CITED
export Copy
Close
Share
  • Twitter
  • Facebook
Similar articles
Copyright © 2024 by Korean Association of Medical Journal Editors. All rights reserved.     E-mail: koreamed@kamje.or.kr