Yonsei Med J.  1988 Jun;29(2):129-138. 10.3349/ymj.1988.29.2.129.

Effect of Carbon Monoxide-Induced Hypoxia on Synaptosomal Uptake and Release of Dopamine in Rat Striatum

• Chai KJ
• Bae SK

• KMID: 1119201
• DOI: http://doi.org/10.3349/ymj.1988.29.2.129

Abstract

We studied the effect of carbon monoxide (CO)-induced hypoxia on synaptosomal uptake and release of dopamine (DA) in rat striatum. When the rats were intoxicated at a blood level of carboxyhemoglobin (HbCO), 60-70% for 3-4hrs, [3H] DA uptake was inhibited as much as 80% of control activity. This suppressed activity remained as long as 12 hrs after termination of the intoxication. After a week recovery period, the suppressed uptake activity was restored completely. When the rats were intoxicated maintaining a blood level of HbCO at 30-40% for 6-7hrs, the uptake was inhibited to 57% of the control actvity and this suppressed activity was restored within 12hrs. For the rats maintaining a blood level of HbCO at 15-25% for 6-7hrs, uptake inhibition was not shown. Acute CO intoxication(at 60-70% of HbCO for 3-4 hrs) caused an increase in K+-stimulated DA release to 147% of the control value. In conclusion, the diminished uptake and increased release of striatal DA in a CO intoxicated brain would cause an extraneuronal accumulation of DA with depletion of intraneuronal DA level, which may play a role in CO-induced hypoxic cell damage.

Keyword

Carbon monoxide-induced hypoxia; dopamine; uptake; release; striatum; synaptosomes

MeSH Terms

Animal
Carbon Monoxide Poisoning/*complications
Corpus Striatum/*ultrastructure
Culture Media
Dopamine/*metabolism
Female
Hypoxia, Brain/chemically induced/*pathology
In Vitro
Male
Rats
Synaptosomes/*metabolism