Yonsei Med J.  1989 Sep;30(3):246-255. 10.3349/ymj.1989.30.3.246.

Anticancer effect of liposome incorporated with methotrexate and antibody against tumor specific surface antigen of rat hepatoma

• Shin YS
• Paik EM
• Ahn YH
• Kim BS
• Kim YS

• KMID: 1097015
• DOI: http://doi.org/10.3349/ymj.1989.30.3.246

Abstract

antibody against tumor specific surface membrane protein was produced by immunizing a New Zealand White rabbit with antigen (66 kDa) prepared from the plasma membrane of rat hepatoma induced by feeding a diet containing 3'-methyl-4-dimethylaminoazobenzene, and was purified by protein A-Sepharose 6MB affinity chromatography. The purified antibody was incorporated into liposomes by a reverse phase evaporation vesicle method in order to prepare a tumor specific anticancer drug carrier. The effect of the antibody against tumor specific antigen was evaluated by comparing the inhibition of DNA synthesis in hepatoma cells with different preparations of methotrexate. Methotrexate encapsulated into liposome showed a stronger inhibitory effect on DNA synthesis (1.4-1.7 times) than free methotrexate. Liposomes having the antibody showed stronger inhibitory effect (3.1 times) on DNA synthesis than free methotrexate group in hepatic nodular area. From these results, it is concluded that tumor specific antibody inserted into liposomal membrane would be recognized by surface antigens which were expressed on the plasma surface membrane of rat hepatoma cells and thereby increase the carrying efficiency of drugs to the target cells. This could be useful in cancer chemotherapy.

Keyword

Liposome drug delivery system; methotrexate; tumor specific surface antigen; hepatoma

MeSH Terms

Animal
Antibodies, Neoplasm
Antigens, Neoplasm/*immunology
Antigens, Surface/*immunology
Drug Carriers
Liposomes
Liver Neoplasms, Experimental/*drug therapy
Male
Methotrexate/*administration and dosage
Rats
Support, Non-U.S. Gov't