J Vet Sci.  2004 Dec;5(4):391-395.

Isolation of cholesterol-lowering lactic acid bacteria from human intestine for probiotic use

• Lim HJ
• Kim SY
• Lee WK

Abstract

Cholesterol-lowering effect of lactic acid bacteria (LAB: Streptococcus, Lactobacillus and Bifidobacterium) is well-known. Thus, we investigated LAB isolated from human intestine on the cholesterol-lowering effect in vitro. Seven Streptococcus (61.1%), 11 Lactobacillus (71.8%) and 7 Bifidobacterium (27.9%) were isolated as acid (pH 2.5 and 3.0) and bile (0.3% oxgall) tolerant strains. Streptococcus HJS-1, Lactobacillus HJL-37 and Bifidobacterium HJB-4 were finally selected as probiotic strains to use through the bile salt hydrolase (BSH) activity assay by using MRS media added taurodeoxycholic acid (TDCA) and the cholesterol-lowering test by using soluble cholesterol containing MRS broth. These studies suggested that the isolated LAB had an excellent hypocholesterolemic effect.

Keyword

Lactic acid bacteria (LAB); probiotics; cholesterol; bile salt hydrolase (BSH)

MeSH Terms

Amidohydrolases/metabolism
Bifidobacterium/*isolation&purification/physiology
Cholesterol/*metabolism
Feces/microbiology
Female
Humans
Intestines/*microbiology
Lactobacillus/*isolation&purification/physiology
Male
Probiotics/*therapeutic use
Streptococcus/*isolation&purification/physiology
Taurodeoxycholic Acid