Exp Mol Med.
2007 Oct;39(5):673-678.
Low-density lipoprotein protects Vibrio vulnificus-induced lethality through blocking lipopolysaccharide action
- Affiliations
-
- 1College of Pharmacy, Medicinal Resources Research Institute, Wonkwang University, Iksan 570-749, Korea.
- 2Department of Biochemistry, Institute of Medical Science,
- 3Department of Microbiology and Immunology, Chonbuk National University Medical School, Jeonju 561-182, Korea. iamtom@chonbuk.ac.kr
- 4Department of Internal Medicine, Chonbuk National University Medical School, Jeonju 561-182, Korea.
- 5Genome Research Center for Enteropathogenic Bacteria, Department of Microbiology, Chonnam National University Medical School, Gwangju 501-746, Korea.
- 6Department of Companion and Laboratory Animal Science, Kongju National University, Yesan 340-702, Korea.
Abstract
- Lipoprotein plays a role in the host defense against bacterial infection, and its serum level has been demonstrated to be an important prognosis factor of survival. We have previously demonstrated that LDL directly inactivates the hemolytic activity of Vibrio vulnificus cytolysin (VVC) in vitro. The object of this study was therefore to examine whether the LDL-mediated inactivation of VVC leads to protection against lethal infection of V. vulnificus in vivo, using wild and VVC-deficient V. vulnificus strains. Unexpectedly, we found that LDL protects mouse lethality induced by VVC-deficient as well as wild V. vulnificus strain. We also demonstrated that LDL blocks V. vulnificus LPS-induced lethality in mice. These results suggest that LDL preferentially act on endotoxin rather than exotoxin in the protection against V. vulnificus-induced mice lethality.
Keyword
MeSH Terms
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Animals
Disease Models, Animal
Female
Humans
Lipopolysaccharides/*antagonists & inhibitors
Lipoproteins, LDL/*pharmacology
Mice
Mice, Inbred ICR
Perforin/antagonists & inhibitors/genetics
Vibrio Infections/prevention & control
Vibrio vulnificus/*drug effects/genetics/*pathogenicity
Virulence/drug effects/genetics/physiology
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