Exp Mol Med.  2001 Jun;33(2):103-105.

An enzymatic method for the detection of human serum albumin

• Javed MU
• Waqar SN

Abstract

Albumin is the most abundant protein in human serum. A dye-binding method is commonly used in clinical laboratories for its estimation using different types of dyes. However, all these dye methods were interfered by a variety of compounds. Here we present a method for the detection of albumin in human serum and other biological fluids. The principle is based on the fact that lactate dehydrogenase isoenzyme-5 (LDH-5) binds specifically to Dextran-Blue (DB). Albumin inhibits the binding of LDH-5 with DB. Absence of LDH activity in DB fraction after gel filtration indicates the presence of albumin in sample and vice versa.

Keyword

albumin; human; LDH-5; serum

MeSH Terms

Chemistry, Clinical/*methods
Chromatography, Gel
Human
Isoenzymes/metabolism
Lactate Dehydrogenase/metabolism
Protein Binding
Sepharose/chemistry
Serum Albumin/*analysis