J Vet Sci.  2010 Dec;11(4):355-357. 10.4142/jvs.2010.11.4.355.

Development of a universal plate-agglutination test for detecting Haemophilus parasuis

• Guo D
• Tang C
• Hai Q
• Shao G
• Yue H

• KMID: 1072187
• DOI: http://doi.org/10.4142/jvs.2010.11.4.355

Abstract

Due to the serovar diversity in Haemophilus (H.) parasuis, it is difficult to develop a universal serological method for detection of this pathogen. Here, we report a universal plate-agglutination test for detecting H. parasuis. Diagnostic antisera were prepared by mixing antisera of serovars 4, 5, 12, 13 and 14 in the optimized ratio. The results of the plate-agglutination test showed that the diagnostic antisera could agglutinate with all 15 reference strains of H. parasuis and 74/75 clinical isolates. Further, the specificity of the method was validated with 22 bacterial strains from 12 related species.

Keyword

diagnostic antisera; Haemophilus parasuis; plate-agglutination

MeSH Terms

Agglutination Tests/*methods
Animals
Cross Reactions
Haemophilus parasuis/isolation & purification/*physiology
Immune Sera/*metabolism
Reproducibility of Results
Sensitivity and Specificity

Figure

• 

  Fig. 1 Visual definition of agglutination reactions. Agglutination reactions photographed while still wet with the colony in the diagnostic antisera. Agglutination activity is defined from uppermost reaction (+++) to negative reaction (-).

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