J Vet Sci.  2010 Dec;11(4):351-353. 10.4142/jvs.2010.11.4.351.

A core set of microsatellite markers for conservation genetics studies of Korean goral (Naemorhedus caudatus) and its cross-species amplification in Caprinae species

Affiliations
  • 1Conservation Genome Resource Bank for Korean Wildlife (CGRB), Research Institute for Veterinary Science, Seoul National University, Seoul 151-742, Korea. hanglee@snu.ac.kr
  • 2BK21 program for Veterinary Science and College of Veterinary Medicine, Seoul National University, Seoul 151-742, Korea. kyungkim@snu.ac.kr
  • 3Grewcock Center for Conservation and Research, Omaha's Henry Doorly Zoo, Omaha, NE 68107, USA.
  • 4Department of Population Biology, Institute of Vertebrate Biology, v.v.i., Academy of Sciences of the Czech Republic, Studenec 122, 675 02 Konesin, Czech Republic.
  • 5Seorak Green Korea United, Sokcho 217-120, Korea.

Abstract

In order to screen microsatellites for conservation genetics studies of the species, a total of 23 microsatellite loci from Korean goral (Naemorhedus caudatus), including 15 previously developed loci and 8 new loci in this study, were tested. Eleven microsatellites were screened and subjected to cross-species amplification using a test panel of four Caprinae species, Japanese serows (Capricornis crispus), Chinese gorals (Naemorhedus goral), Northern chamois (Rupicapra rupicapra) and domestic goats (Capra hircus). In addition, all eleven microsatellites (SY3A, SY12A, SY12B, SY48, SY58, SY71, SY76, SY84, SY84B, SY112, and SY129) satisfied the criteria to be a core set of microsatellites. This core set of microsatellites and cross-species amplification of Korean goral microsatellites were found to be helpful for high-resolution studies for conservation and management of Korean goral and other endangered Caprinae species.

Keyword

genetic diversity; Korean goral; microsatellites; Naemorhedus caudatus

MeSH Terms

Animals
Conservation of Natural Resources
Genetic Variation
Hybridization, Genetic/*genetics
Microsatellite Repeats/*genetics
Republic of Korea
Ruminants/*genetics

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