J Korean Med Sci.  1995 Jun;10(3):155-163. 10.3346/jkms.1995.10.3.155.

Effect of growth factors on the expression of proto-oncogenes c-fos and c-myc in FRTL-5 cell line

• Yoon HY
• Oh SK
• Yi KH
• Cho BY
• Koh CS

• KMID: 1054338
• DOI: http://doi.org/10.3346/jkms.1995.10.3.155

Abstract

This study was performed to prove the hypothesis that oncogene expressions would have the same patterns with those of cellular growth to growth factors in FRTL-5 cells. Ribonucleic acids of FRTL-5 were extracted at 15', 30', 60' and 120' after administration of growth factors to quiescent FRTL-5, and blotted to the nitrocellulose membrane. They were hybridized with radiolabelled c-fos, c-myc and beta-actin probes. Hybridized dot blots were autoradiographed and the amount of radioactivity was measured by densitometry. Densitometric readings were used as the indices of oncogene expressions. Expressions of c-fos and c-myc were more prominent in combined administrations of TSH (10 mU/ml) and IGF-I (100 ng/ml) or IgG of Graves' disease (Graves' IgG; 1 mg/ml) and IGF-I than in combined administration of TSH and Graves' IgG. IgG of primary myxedema suppressed oncogene expressions by TSH or Graves' IgG, but not by IGF-I. From the above results, it was suggested that expressions of c-fos and c-myc to growth factors would have similar patterns with those of cell growth to growth factors in FRTL-5, and the actions of TSH and Graves' IgG would be manifested through same signal transduction system, but IGF-I would be manifested by its own.

Keyword

Growth factors; c-fos; c-myc; signal transduction system

MeSH Terms

Animal
Cell Division/drug effects/genetics
Cell Line/cytology/physiology
Gene Expression/drug effects/immunology
Graves' Disease/immunology
Growth Substances/genetics/*pharmacology
Immunoglobulin G/pharmacology
Insulin-Like Growth Factor I/pharmacology
Myxedema/immunology
Proto-Oncogene Proteins c-fos/*genetics
Proto-Oncogene Proteins c-myc/*genetics
RNA/analysis
Rats
Rats, Inbred F344
Support, Non-U.S. Gov't
Thyroid Gland/cytology
Thyrotropin/pharmacology
Time Factors