Korean J Parasitol.  2001 Jun;39(2):119-132. 10.3347/kjp.2001.39.2.119.

Immunoelectron microscopic localization of partially purified antigens in adult Paragonimus iloktsuenesis

• Lee OR
• Chung PR

• KMID: 1039034
• DOI: http://doi.org/10.3347/kjp.2001.39.2.119

Abstract

An immunoelectron microscopy employing immunogold labeling method was performed to detect tissue origin of D1 fraction (D1A) among 5 antigenic protein fractions partially purified by DEAE-anion exchange chromatography from water-soluble crude antigen (PIWA) of adult Paragonimus iloktsuenensis. Immune reactions of adult worm tissues with rabbit serum immunoglobulin immunized with crude antigen (PI-Ig) and D1 antigen (D1-Ig), as well as rat serum immunoglobulin infected with P. iloktsuenensis were observed. D1A showed strong antigenicity in the intestinal epithelium of the worms during the early infection period of 2-4 weeks after infection. The vitellaria also showed stronger antigenicity than the other tissue sites in immune reaction of tissues against all immunoglobulins from 4 to 33 weeks after vitelline development. Therefore, it is suggested that D1A was mainly originated from the intestinal epithelial tissues before the development of vitelline gland of the parasites. Immuno-reactivity of two immunoglobulins (PI-Ig, D1-Ig) was significantly different in intestinal epithelial cytoplasmic protrusions (CP) and intestinal epithelial secretory granules (SG). In the experimental group with D1-Ig, gold particles were labeled significantly in CP than in SG when compared to the PI-Ig group. Thus, the major antigenic materials in D1 antigen having a strong antigenicity in the early infection period was considered to be originated from the intestinal epithelial tissue.

MeSH Terms

Animals
Antigens, Helminth/*analysis/isolation & purification
Chromatography, Ion Exchange
Immunohistochemistry/methods
Microscopy, Immunoelectron
Paragonimus/*immunology
Support, Non-U.S. Gov't