J Korean Med Sci.  2000 Aug;15(Suppl):S38-S39. 10.3346/jkms.2000.15.S.S38.

Fluid and amylase secretion by perfused parotid gland: physio-morphological approach

• Murakami M
• Yoshimura
• Sugiya H
• Segawa A
• Loffredo F
• Testa-Riva F
• Riva A

• KMID: 1026945
• DOI: http://doi.org/10.3346/jkms.2000.15.S.S38

Abstract

Whole gland perfusion technique was applied to rat parotid glands to assess whether amylase affects fluid secretion. Control perfusion without any secretagogue evoked no spontaneous secretion. Carbachol (CCh 1 microM) induced both amylase and fluid secretion with distinctive kinetics. Fluid secretion occurred constantly around 60 microL/g-min, whereas amylase secretion exhibited an initial peak, followed by a rapid decrease to reach a plateau. Isoproterenol (Isop 1 microM) alone did not induce fluid secretion although it evoked amylase secretion as measured in isolated perfused acini. Addition of Isop during CCh stimulation evoked a rapid and large rise in amylase secretion accompanied by small increase in oxygen consumption. Morphological observations carried out by HR SEM and TEM revealed exocytotic profiles following Isop stimulation. CCh stimulation alone seldom showed exocytotic profiles, suggesting a low incidence of amylase secretion during copious fluid secretion. Combined stimulation of CCh and Isop induced both vacuolation and exocytosis along intercellular canaliculi. These findings suggest that control of salivary fluid secretion is independent of the amylase secretion system induced by CCh and/or Isop.

Keyword

Amylases Secretion; Fluid Secretion; Salivary Glands; Whole Gland Perfusion; HR-SEM

MeSH Terms

Amylases/metabolism*
Animal
Carbachol/pharmacology
Cholinergic Agonists/pharmacology
In Vitro
Isoproterenol/pharmacology
Male
Microscopy, Electron
Microscopy, Electron, Scanning
Oxygen Consumption/physiology
Oxygen Consumption/drug effects
Parotid Gland/ultrastructure
Parotid Gland/secretion*
Parotid Gland/enzymology*
Perfusion
Rats
Rats, Wistar
Saliva/metabolism*
Sympathomimetics/pharmacology