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Developing species-specific quantitative real-time polymerase chain reaction primers for detecting Lautropia mirabilis

Park SN, Kook JK

This study aimed to develop Lautropia mirabilis-specific quantitative real-time polymerase chain reaction (qPCR) primers based on the sequence of DNA-directed RNA polymerase subunit beta gene. The PrimerSelect program was used...
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Clinical efficacy of respiratory virus detection by using the FilmArray method in children admitted with respiratory infection

Lee HJ, Park JH, Kim JM, Kim JH, Baek HS

Purpose: Respiratory virus infection is a common cause of hospitalization in children. Rapid testing for respiratory viruses, such as the FilmArray method, can be clinically useful. However, insufficient evidence exists...
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Successful Treatment of Mycoplasma hominis Meningitis, Diagnosed Using Real-Time Polymerase Chain Reaction, with Ciprofloxacin in a Neonate

Gwon I, Song WS, Sohn YH, Kim SY

Mycoplasma hominis can cause life-threatening central nervous system infections in neonates following intrauterine infection or during delivery. In newborns, the dia gnosis and treatment of M. hominis meningitis are challenging,...
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Expression and Significance of MicroRNA155 in Serum of Patients with Cerebral Small Vessel Disease

Guo Y, Li D, Li J, Yang N, Wang D

Objective : This study aimed to investigate the changes and significance of microRNA155 levels in serum of patients with cerebral small vessel disease (CSVD). Methods : Thirty patients with CSVD who...
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Role of IL-17A in Chronic Rhinosinusitis With Nasal Polyp

Ryu G, Bae JS, Kim JH, Kim EH, Lyu L, Chung YJ, Mo JH

PURPOSE: Th17-associated inflammation is increased in chronic rhinosinusitis with nasal polyp (CRSwNP), and is associated with disease severity and steroid resistance. Overexpressed interleukin (IL)-17A affects CRSwNP by tissue remodeling, eosinophilic...
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miR-155, miR-191, and miR-494 as diagnostic biomarkers for oral squamous cell carcinoma and the effects of Avastin on these biomarkers

Emami N, Mohamadnia A, Mirzaei M, Bayat M, Mohammadi F, Bahrami N

Objectives: Oral squamous cell carcinoma (OSCC) is one of the most common types of head and neck cancer. MicroRNAs, as new biomarkers, are recommended for diagnosis and treatment of different...
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Combined Detection of Serum MiR-221-3p and MiR-122-5p Expression in Diagnosis and Prognosis of Gastric Cancer

Zhang Y, Huang H, Zhang Y, Liao N

PURPOSE: To investigate the clinical value of serum miR-221-3p and miR-122-5p expression levels in the diagnosis and prognosis of gastric cancer. MATERIALS AND METHODS: Serum samples from 141 gastric cancer cases...
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Comparison of Multiplex Real-Time Polymerase Chain Reaction Assays for Detection of Respiratory Viruses in Nasopharyngeal Specimens

Uwizeyimana JD, Kim MK, Kim D, Byun JH, Yong D

BACKGROUND: Respiratory tract infections are major public health threats, and the identification of their causative microbes helps clinicians to initiate timely and appropriate antimicrobial therapy and prevent the secondary spread...
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Real-time PCR quantification of 9 periodontal pathogens in saliva samples from periodontally healthy Korean young adults

Choi H, Kim E, Kang J, Kim HJ, Lee JY, Choi J, Joo JY

PURPOSE: Few studies have examined periodontal pathogens from saliva samples in periodontally healthy young adults. The purposes of this study were to determine the prevalence of periodontopathic bacteria and to...
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Validity of the New Caries Activity Test using Real-Time Polymerase Chain Reaction

Kwon D, Kim H, Nam O, Kim M, Choi S, Kim K, Lee H

Periogen is a new caries activity test using real-time polymerase chain reaction. The aim of this study was to assess the validity of Periogen by evaluating the correlation with dmft,...
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Genetic identification and serological evaluation of commercial inactivated foot-and-mouth disease virus vaccine in pigs

Je SH, Kwon T, Yoo SJ, Lee DU, Seo SW, Byun JJ, Shin JY, Lyoo YS

Vaccination is considered a frequently used tool to prevent and control foot-and-mouth disease (FMD). However, the effectiveness of conventional FMD virus (FMDV) vaccines in pigs has been controversial because the...
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Evaluation of EZplex MTBC/NTM Real-Time PCR kit: diagnostic accuracy and efficacy in vaccination

Lee S, Hwang KA, Ahn JH, Nam JH

PURPOSE: Tuberculosis (TB) is mainly caused by Mycobacterium tuberculosis, which is a pathogenic mycobacterial species grouped under Mycobacterium tuberculosis complex (MTBC) with four other pathogenic mycobacterial species. The mycobacteria not...
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Characterization of the fecal microbiota differs between age groups in Koreans

Kook SY, Kim Y, Kang B, Choe YH, Kim YH, Kim S

BACKGROUND/AIMS: Tens of trillions of microorganisms constitute the gut microbiota of the human body. The microbiota plays a critical role in maintaining host immunity and metabolism. Analyses of the gut...
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Study of Bacteria Associated with Dental Caries Using a 3 Tone Disclosing Agent

Lee J, Park H, Lee J, Seo H, Lee S

The aim of this study was to determine the efficacy of a 3 tone plaque disclosing gel in assessing the risk of caries related to the population of Streptococcus mutans,...
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Quantitative Analysis of Oral Pathogenic Bacteria according to Smoking Using Real-Time PCR

Jeon ES, Heo HJ, Ko HJ

This study investigates the relationship between smoking and periodontal disease through quantitative analysis of intra-buccal oral pathogenic bacteria detected in smokers and aims to yield objective baseline data for applications...
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Shorter Telomere Length Is Associated with Increased Breast Cancer Risk in a Chinese Han Population: A Case-Control Analysis

Wang Z, Zhang Z, Guo Y, Shui H, Liu G, Jin T, Wang H

PURPOSE: The aim of this study was to investigate the association of telomere length with breast cancer risk. We simultaneously explored the association between telomerase reverse transcriptase gene polymorphisms and...
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Comparison of PANA RealTyper HPV Kit with AdvanSure HPV GenoBlot Assay for Human Papillomavirus Genotyping

Kim YH, Chung HS, Lee M

BACKGROUND: The PANA RealTyper HPV kit (PANAGENE, Korea; PANA RealTyper) was developed to genotype human papillomavirus (HPV) and was based on multiplex real-time PCR amplification and melting curve analysis. In...
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Diagnostic Accuracy and Detection Rate of Real-Time PCR for Detection of Group B Streptococcal Colonization in Pregnant Women: Systemic Review of Literature and Meta-Analysis

Park SY, Kim SY, Choi WJ, Kim SH, Hong SG

BACKGROUND: Group B streptococcus (Streptococcus agalactiae, GBS) was reported as a major cause of neonatal infection and death. To prevent vertical transmission, CDC recommended that all women in week 35-37...
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Practical Aspects of Cytomegalovirus DNA Quantitative PCR

Chang J, Hwang SH, Kim MN, Sung H

Human cytomegalovirus (CMV) is a clinically important pathogen that causes significant morbidity and mortality in immunocompromised patients and is typically monitored using real-time polymerase chain reaction (real-time PCR). International standards...
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Molecular-level evaluation of selected periodontal pathogens from subgingival regions in canines and humans with periodontal disease

Gołyńska M, Polkowska , Bartoszcze-Tomaszewska M, Sobczyńska-Rak A, Matuszewski L

Dogs commonly serve as a model for various human conditions, including periodontal diseases. The aim of this study was to identify the anaerobic bacteria that colonize the subgingival areas in...
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